Effect of Ad.DF3-tk Transduction and GCV Treatment on the Growth of Normal Human Hematopoietic Progenitor Cells
| Cell . | Treatment . | BFU-E* . | CFU-GM* . |
|---|---|---|---|
| BM MNC† | Control | 165 ± 0 | 400 ± 0 |
| Ad.DF3-tk(MOI = 10) | 225 ± 20 | 375 ± 15 | |
| GCV (50 μmol/L) | 205 ± 10 | 350 ± 10 | |
| Ad.DF3-tk (MOI = 10) + GCV (50 μmol/L) | 220 ± 0 | 440 ± 0 | |
| RPMI 8226‡ | Control | 1-153 | 1-153 |
| Ad.DF3-tk(MOI = 10) + GCV (50 μmol/L) | 240 ± 351-155 | 315 ± 451-155 |
| Cell . | Treatment . | BFU-E* . | CFU-GM* . |
|---|---|---|---|
| BM MNC† | Control | 165 ± 0 | 400 ± 0 |
| Ad.DF3-tk(MOI = 10) | 225 ± 20 | 375 ± 15 | |
| GCV (50 μmol/L) | 205 ± 10 | 350 ± 10 | |
| Ad.DF3-tk (MOI = 10) + GCV (50 μmol/L) | 220 ± 0 | 440 ± 0 | |
| RPMI 8226‡ | Control | 1-153 | 1-153 |
| Ad.DF3-tk(MOI = 10) + GCV (50 μmol/L) | 240 ± 351-155 | 315 ± 451-155 |
Bone marrow MNCs (2 × 105 cells/well) without (
) or with (
) contaminating (1%) RPMI 8226 MM cells were transduced in duplicate with Ad. DF3-tk at MOI = 10 for 2 hours, washed out for 10 hours, and then treated with GCV (50 μmol/L) for 24 hours. CFU-GM and BFU-E colonies were enumerated after 14 days of culture in Iscove’s methylcellulose medium.
Expressed as colonies per 1 × 106 cells.
RPMI 8226 MM cells overgrew as a monolayer.
There were no visible RPMI 8226 MM cells.