Expression of Human CD45 Antigen in NOD/SCID Recipients of AML Cells Sorted for Coexpression of CD34 and CD71 (CD34+ patients)
| Patient No. . | Phenotype . | % Nucleated Cells . | No. Cells Injected . | % CD45+ BM . | FISH Analysis on Colonies . | ||
|---|---|---|---|---|---|---|---|
| Positive . | Undetermined . | Negative . | |||||
| 3 | Unsorted | 106 | 15 | 3/4 | 1/4 | ||
| CD34+/CD71+ | 1.8 | 1.5 × 105 | 0 | ||||
| CD34+/CD71− | 45.8 | 106 | 74 | 2/4 | 1/4 | 1/4 | |
| CD34− | 52.4 | 106 | 0 | ||||
| 4 | Unsorted | 106 | 24 | ||||
| Unsorted | 5 × 105 | 43 | 5/6 | 1/6 | |||
| Unsorted | 105 | 5 | |||||
| Unsorted | ≤5 × 104 | 0 | |||||
| CD34+/CD71+ | 24.5 | ≤5 × 105 | 0 | ||||
| CD34+/CD71− | 3.9 | 2.5 × 105 | 5 | 1/5 | 2/5 | 2/5 | |
| CD34+/CD71− | ≤5 × 104 | 0 | |||||
| CD34− | 71.6 | ≤5 × 105 | 0 | ||||
| 5 | Unsorted | 106 | 0 | ||||
| CD34+/CD71+ | 62.6 | ≤106 | 0 | ||||
| CD34+/CD71− | 13.2 | 105 | 0.2 | ||||
| CD34+/CD71− | 5 × 104 | 0.2 | |||||
| CD34+/CD71− | 104 | 0.3 | |||||
| CD34− | 24.2 | 1.4 × 106 | 0 | ||||
| 11 | Unsorted | 106 | 0.1 | ||||
| CD34+/CD71+ | 8.8 | 105 | 0 | ||||
| CD34+/CD71+ | 5.4 × 104 | 0 | |||||
| CD34+/CD71− | 39.1 | 2 × 105 | 0.2 | ||||
| CD34+/CD71− | 6 × 104 | 0.2 | |||||
| CD34+/CD71− | 5 × 104 | 0 | |||||
| CD34− | 52.1 | ≤3 × 105 | 0 | ||||
| 12 | Unsorted | 5 × 105 | 0.2 | ||||
| Unsorted | ≤105 | 0 | |||||
| CD34+/CD71+ | 5.1 | 106 | 0 | ||||
| CD34+/CD71− | 67.2 | 105 | 3 | ||||
| CD34+/CD71− | ≤5 × 104 | 0 | |||||
| CD34− | 27.7 | ≤5 × 105 | 0 | ||||
| Patient No. . | Phenotype . | % Nucleated Cells . | No. Cells Injected . | % CD45+ BM . | FISH Analysis on Colonies . | ||
|---|---|---|---|---|---|---|---|
| Positive . | Undetermined . | Negative . | |||||
| 3 | Unsorted | 106 | 15 | 3/4 | 1/4 | ||
| CD34+/CD71+ | 1.8 | 1.5 × 105 | 0 | ||||
| CD34+/CD71− | 45.8 | 106 | 74 | 2/4 | 1/4 | 1/4 | |
| CD34− | 52.4 | 106 | 0 | ||||
| 4 | Unsorted | 106 | 24 | ||||
| Unsorted | 5 × 105 | 43 | 5/6 | 1/6 | |||
| Unsorted | 105 | 5 | |||||
| Unsorted | ≤5 × 104 | 0 | |||||
| CD34+/CD71+ | 24.5 | ≤5 × 105 | 0 | ||||
| CD34+/CD71− | 3.9 | 2.5 × 105 | 5 | 1/5 | 2/5 | 2/5 | |
| CD34+/CD71− | ≤5 × 104 | 0 | |||||
| CD34− | 71.6 | ≤5 × 105 | 0 | ||||
| 5 | Unsorted | 106 | 0 | ||||
| CD34+/CD71+ | 62.6 | ≤106 | 0 | ||||
| CD34+/CD71− | 13.2 | 105 | 0.2 | ||||
| CD34+/CD71− | 5 × 104 | 0.2 | |||||
| CD34+/CD71− | 104 | 0.3 | |||||
| CD34− | 24.2 | 1.4 × 106 | 0 | ||||
| 11 | Unsorted | 106 | 0.1 | ||||
| CD34+/CD71+ | 8.8 | 105 | 0 | ||||
| CD34+/CD71+ | 5.4 × 104 | 0 | |||||
| CD34+/CD71− | 39.1 | 2 × 105 | 0.2 | ||||
| CD34+/CD71− | 6 × 104 | 0.2 | |||||
| CD34+/CD71− | 5 × 104 | 0 | |||||
| CD34− | 52.1 | ≤3 × 105 | 0 | ||||
| 12 | Unsorted | 5 × 105 | 0.2 | ||||
| Unsorted | ≤105 | 0 | |||||
| CD34+/CD71+ | 5.1 | 106 | 0 | ||||
| CD34+/CD71− | 67.2 | 105 | 3 | ||||
| CD34+/CD71− | ≤5 × 104 | 0 | |||||
| CD34− | 27.7 | ≤5 × 105 | 0 | ||||
The remainder of cells to 100% were CD45−.