Expression of Human CD45 Antigen in NOD/SCID Recipients of AML Cells Sorted for Coexpression of CD34 and CD71 (CD34− patients)
| Patient No. . | Phenotype . | % Nucleated Cells . | No. Cells Injected . | % CD45+ BM . | FISH Analysis on Colonies . | ||
|---|---|---|---|---|---|---|---|
| Positive . | Undetermined . | Negative . | |||||
| 2 | Unsorted | 106 | 55 | 3/4 | 1/4 | ||
| Unsorted | 5 × 105 | 2 | |||||
| Unsorted | ≤105 | 0 | |||||
| CD34+/CD71+ | 0.02 | 7 × 103 | 0 | ||||
| CD34+/CD71− | 1.1 | 2 × 104 | 0 | ||||
| CD34+/CD71− | 103 | 1 | |||||
| CD34− | 98.88 | 4 × 105 | 37 | ||||
| CD34− | ≤104 | 0 | |||||
| 8 | Unsorted | 106 | 96 | 4/4 | |||
| Unsorted | 5 × 105 | 92 | 3/3 | ||||
| Unsorted | 105 | 66 | 4/4 | ||||
| Unsorted | 5 × 104 | 26 | |||||
| ≤104 | 0 | ||||||
| CD34+/CD71+ | 0.4 | 7 × 103 | 0 | ||||
| CD34+/CD71− | 0.01 | 4 × 103 | 11 | 4/4 | |||
| CD34− | 99.59 | 106 | 31 | 3/3 | |||
| CD34− | 5 × 105 | 54 | 5/5 | ||||
| CD34− | 105 | 11 | 3/3 | ||||
| CD34− | ≤5 × 104 | 0 | |||||
| 15 | Unsorted | 106 | 24 | ||||
| Unsorted | 5 × 105 | 9 | |||||
| Unsorted | 105 | 1 | |||||
| Unsorted | ≤104 | 0 | |||||
| CD34+/CD71+ | 0.01 | 103 | 0 | ||||
| CD34+/CD71− | 0.1 | 5 × 103 | 27 | ||||
| CD34− | 99.89 | ≤106 | 0 | ||||
| 18 | Unsorted | ≤106 | 0 | ||||
| CD34+/CD71+ | 0.3 | ≤5 × 104 | 0 | ||||
| CD34+/CD71− | 0.2 | 5 × 104 | 0.1 | ||||
| CD34+/CD71− | 104 | 0.2 | |||||
| CD34+/CD71− | 104 | 0.1 | |||||
| CD34− | 99.5 | ≤106 | 0 | ||||
| Patient No. . | Phenotype . | % Nucleated Cells . | No. Cells Injected . | % CD45+ BM . | FISH Analysis on Colonies . | ||
|---|---|---|---|---|---|---|---|
| Positive . | Undetermined . | Negative . | |||||
| 2 | Unsorted | 106 | 55 | 3/4 | 1/4 | ||
| Unsorted | 5 × 105 | 2 | |||||
| Unsorted | ≤105 | 0 | |||||
| CD34+/CD71+ | 0.02 | 7 × 103 | 0 | ||||
| CD34+/CD71− | 1.1 | 2 × 104 | 0 | ||||
| CD34+/CD71− | 103 | 1 | |||||
| CD34− | 98.88 | 4 × 105 | 37 | ||||
| CD34− | ≤104 | 0 | |||||
| 8 | Unsorted | 106 | 96 | 4/4 | |||
| Unsorted | 5 × 105 | 92 | 3/3 | ||||
| Unsorted | 105 | 66 | 4/4 | ||||
| Unsorted | 5 × 104 | 26 | |||||
| ≤104 | 0 | ||||||
| CD34+/CD71+ | 0.4 | 7 × 103 | 0 | ||||
| CD34+/CD71− | 0.01 | 4 × 103 | 11 | 4/4 | |||
| CD34− | 99.59 | 106 | 31 | 3/3 | |||
| CD34− | 5 × 105 | 54 | 5/5 | ||||
| CD34− | 105 | 11 | 3/3 | ||||
| CD34− | ≤5 × 104 | 0 | |||||
| 15 | Unsorted | 106 | 24 | ||||
| Unsorted | 5 × 105 | 9 | |||||
| Unsorted | 105 | 1 | |||||
| Unsorted | ≤104 | 0 | |||||
| CD34+/CD71+ | 0.01 | 103 | 0 | ||||
| CD34+/CD71− | 0.1 | 5 × 103 | 27 | ||||
| CD34− | 99.89 | ≤106 | 0 | ||||
| 18 | Unsorted | ≤106 | 0 | ||||
| CD34+/CD71+ | 0.3 | ≤5 × 104 | 0 | ||||
| CD34+/CD71− | 0.2 | 5 × 104 | 0.1 | ||||
| CD34+/CD71− | 104 | 0.2 | |||||
| CD34+/CD71− | 104 | 0.1 | |||||
| CD34− | 99.5 | ≤106 | 0 | ||||
The remainder of cells to 100% were CD45−.