Table 6. Expression of Human CD45... | American Society of Hematology

Table 6.

Expression of Human CD45 Antigen in NOD/SCID Recipients of AML Cells Sorted for Coexpression of CD34, HLA-DR, and CD71

Patient No.	Phenotype	% Nucleated Cells	No. Cells Injected	% CD45⁺ BM	FISH Analysis on Colonies
Patient No.	Phenotype	% Nucleated Cells	No. Cells Injected	% CD45⁺ BM	Positive	Undetermined	Negative
4	Unsorted		10⁶	16
	Unsorted		5 × 10⁵	41	3/5		2/5
	CD34⁺/CD71⁺/DR⁺	21.95	NA
	CD34⁺/CD71⁺/DR⁻	4.36	10⁴	0
	CD34⁺/CD71⁻/DR⁺	1.14	4 × 10⁴	0
			9 × 10³	0
	CD34⁺/CD71⁻/DR⁻	0.56	5 × 10³	39	1/4	3/4
			500	0
	CD34⁻	71.99	≤10⁶	0
8	Unsorted		10⁶	97	5/5
	CD34⁺/DR⁺	0.1	8 × 10³	0
			5 × 10³	0
	CD34⁺/DR⁻	0.01	3 × 10³	18	5/5
	CD34⁺/CD71⁺/DR⁺	0.06	NA
	CD34⁺/CD71⁺/DR⁻	0.008	NA
	CD34⁺/CD71⁻/DR⁺	0.04	5 × 10³	0
	CD34⁺/CD71⁻/DR⁻	0.002	2272	32	5/5
			400	4	3/3
	CD34⁻	99.89	10⁶	98	4/4
			10⁵	29	4/4
9	Unsorted		10⁶	0.2
	CD34⁺/DR⁺	4.6	7 × 10⁴	0
	CD34⁺/DR⁻	4.5	10⁵	0
			8 × 10⁴	0
			7 × 10⁴	0
	CD34⁺/CD71⁺/DR⁺	3.7	NA
	CD34⁺/CD71⁺/DR⁻	2.9	NA
	CD34⁺/CD71⁻/DR⁺	0.9	8 × 10⁴	0
			4 × 10⁴	0
	CD34⁺/CD71⁻/DR⁻	1.6	3.7 × 10⁴	1
	CD34⁻	90.9	≤10⁶	0
13	Unsorted		10⁶	0.1
	CD34⁺/DR⁺	10.3	5 × 10⁵	0
			3.5 × 10⁵	0
	CD34⁺/DR⁻	0.07	5 × 10⁵	4	3/3
			10⁵	0
	CD34⁺/CD71⁺/DR⁺	9.6	NA
	CD34⁺/CD71⁺/DR⁻	0.05	NA
	CD34⁺/CD71⁻/DR⁺	0.7	2.5 × 10⁴	0
	CD34⁺/CD71⁻/DR⁻	0.02	10⁵	13	3/3
			2 × 10⁴	3	3/3
	CD34⁻	89.63	5 × 10⁶	0
15	Unsorted		10⁶	36
	CD34⁺/DR⁺	0.11	1.7 × 10⁴	0
	CD34⁺/DR⁻	0.09	5.6 × 10³	29
	CD34⁺/CD71⁺/DR⁺	0.02	NA
	CD34⁺/CD71⁺/DR⁻	0.01	NA
	CD34⁺/CD71⁻/DR⁺	0.09	5 × 10³	0
	CD34⁺/CD71⁻/DR⁻	0.08	3.7 × 10³	40
			2.5 × 10³	19
	CD34⁻	99.8	10⁶	0
17	Unsorted		10⁶	13
			5 × 10⁵	11
			10⁵	8
	CD34⁺/DR⁺	1.3	2 × 10⁵	0
	CD34⁺/DR⁻	5.3	3 × 10⁵	38
	CD34⁻	93.4	10⁶	0
			6 × 10⁵	0

Patient No.	Phenotype	% Nucleated Cells	No. Cells Injected	% CD45⁺ BM	FISH Analysis on Colonies
Patient No.	Phenotype	% Nucleated Cells	No. Cells Injected	% CD45⁺ BM	Positive	Undetermined	Negative
4	Unsorted		10⁶	16
	Unsorted		5 × 10⁵	41	3/5		2/5
	CD34⁺/CD71⁺/DR⁺	21.95	NA
	CD34⁺/CD71⁺/DR⁻	4.36	10⁴	0
	CD34⁺/CD71⁻/DR⁺	1.14	4 × 10⁴	0
			9 × 10³	0
	CD34⁺/CD71⁻/DR⁻	0.56	5 × 10³	39	1/4	3/4
			500	0
	CD34⁻	71.99	≤10⁶	0
8	Unsorted		10⁶	97	5/5
	CD34⁺/DR⁺	0.1	8 × 10³	0
			5 × 10³	0
	CD34⁺/DR⁻	0.01	3 × 10³	18	5/5
	CD34⁺/CD71⁺/DR⁺	0.06	NA
	CD34⁺/CD71⁺/DR⁻	0.008	NA
	CD34⁺/CD71⁻/DR⁺	0.04	5 × 10³	0
	CD34⁺/CD71⁻/DR⁻	0.002	2272	32	5/5
			400	4	3/3
	CD34⁻	99.89	10⁶	98	4/4
			10⁵	29	4/4
9	Unsorted		10⁶	0.2
	CD34⁺/DR⁺	4.6	7 × 10⁴	0
	CD34⁺/DR⁻	4.5	10⁵	0
			8 × 10⁴	0
			7 × 10⁴	0
	CD34⁺/CD71⁺/DR⁺	3.7	NA
	CD34⁺/CD71⁺/DR⁻	2.9	NA
	CD34⁺/CD71⁻/DR⁺	0.9	8 × 10⁴	0
			4 × 10⁴	0
	CD34⁺/CD71⁻/DR⁻	1.6	3.7 × 10⁴	1
	CD34⁻	90.9	≤10⁶	0
13	Unsorted		10⁶	0.1
	CD34⁺/DR⁺	10.3	5 × 10⁵	0
			3.5 × 10⁵	0
	CD34⁺/DR⁻	0.07	5 × 10⁵	4	3/3
			10⁵	0
	CD34⁺/CD71⁺/DR⁺	9.6	NA
	CD34⁺/CD71⁺/DR⁻	0.05	NA
	CD34⁺/CD71⁻/DR⁺	0.7	2.5 × 10⁴	0
	CD34⁺/CD71⁻/DR⁻	0.02	10⁵	13	3/3
			2 × 10⁴	3	3/3
	CD34⁻	89.63	5 × 10⁶	0
15	Unsorted		10⁶	36
	CD34⁺/DR⁺	0.11	1.7 × 10⁴	0
	CD34⁺/DR⁻	0.09	5.6 × 10³	29
	CD34⁺/CD71⁺/DR⁺	0.02	NA
	CD34⁺/CD71⁺/DR⁻	0.01	NA
	CD34⁺/CD71⁻/DR⁺	0.09	5 × 10³	0
	CD34⁺/CD71⁻/DR⁻	0.08	3.7 × 10³	40
			2.5 × 10³	19
	CD34⁻	99.8	10⁶	0
17	Unsorted		10⁶	13
			5 × 10⁵	11
			10⁵	8
	CD34⁺/DR⁺	1.3	2 × 10⁵	0
	CD34⁺/DR⁻	5.3	3 × 10⁵	38
	CD34⁻	93.4	10⁶	0
			6 × 10⁵	0

The remainder of cells to 100% were CD45⁻.

Abbreviation: NA, data is not available.

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