Consensus Sequences for Putative Transcription Factors Found in Endoglin and Other Endothelial Genes Promoters
| Factor . | Consensus Sequence . | Endoglin Sequence (position)* . | Endothelial Genes . | Reference . | |
|---|---|---|---|---|---|
| AP-2 | CCC(A/C)N(G/C)(G/C)(G/C) | CCCAGCGC CCCCTCGG GGGATGGG GGGTGGGG CCCACCCC | (−397) (−256) (−176) R (−167) R (−126) | GPIIb, vWF, PF4, P-selectin, E-selectin, VCAM-1, ICAM-1 | 33 |
| CCCATCCC | (−108) | ||||
| CCCAGGGC | (−103) | ||||
| CCCCACCC | (−94) | ||||
| CCCAGCCC | (−37) | ||||
| GCCCTGGG | (+68) R | ||||
| CCCTGGGG | (+69) R | ||||
| CCCCTCCC | (+119) | ||||
| CCCCGCCC | (+124) | ||||
| CCCAGCCC | (+154) | ||||
| CCCCTCGG | (+188) | ||||
| CCCCTCGG | (+247) | ||||
| CCCACGCC | (+268) | ||||
| CCCAGCGC | (+322) | ||||
| Ets | (G/C)(A/C)GGA(A/T)G(T/C) | GtTTCCTC CAGGAAcT ACTTCCTC AGGAAGC AtTTCCTG | (−347) R (−294) (−68) R (−21) (−12) R | GPIIb, vWF, PF4, P-selectin, E-selectin, VLA-4, ICAM-1 | 34 |
| GATA | (A/T)GATA(A/G) | GATAA | (+315) | GPIIb, vWF, PF4, Endothelin-1, VLA-4, PECAM-1 | 35 |
| NFκB | GGG(A/G)N2(C/T)(C/T)CC | GGGGCTCCC GGGAGCTCCC | (−218) D/R (+27) D/R | E-selectin, VCAM-1, ICAM-1, PECAM-1 | 36 |
| Sp1 | (T/G)(A/G)GGC(T/G)(A/G)(A/G)(T/G) | ACCAGCCT CCCAGCCCC CCCCGCCCA CCAGCCCA CCCAGCCCC | (−194) R (−37) R (+124) R (+150) R (+154) R | GPIIb, vWF, PF4, Endothelin-1, P-selectin, E-selectin, ICAM-1 | 37 |
| Factor . | Consensus Sequence . | Endoglin Sequence (position)* . | Endothelial Genes . | Reference . | |
|---|---|---|---|---|---|
| AP-2 | CCC(A/C)N(G/C)(G/C)(G/C) | CCCAGCGC CCCCTCGG GGGATGGG GGGTGGGG CCCACCCC | (−397) (−256) (−176) R (−167) R (−126) | GPIIb, vWF, PF4, P-selectin, E-selectin, VCAM-1, ICAM-1 | 33 |
| CCCATCCC | (−108) | ||||
| CCCAGGGC | (−103) | ||||
| CCCCACCC | (−94) | ||||
| CCCAGCCC | (−37) | ||||
| GCCCTGGG | (+68) R | ||||
| CCCTGGGG | (+69) R | ||||
| CCCCTCCC | (+119) | ||||
| CCCCGCCC | (+124) | ||||
| CCCAGCCC | (+154) | ||||
| CCCCTCGG | (+188) | ||||
| CCCCTCGG | (+247) | ||||
| CCCACGCC | (+268) | ||||
| CCCAGCGC | (+322) | ||||
| Ets | (G/C)(A/C)GGA(A/T)G(T/C) | GtTTCCTC CAGGAAcT ACTTCCTC AGGAAGC AtTTCCTG | (−347) R (−294) (−68) R (−21) (−12) R | GPIIb, vWF, PF4, P-selectin, E-selectin, VLA-4, ICAM-1 | 34 |
| GATA | (A/T)GATA(A/G) | GATAA | (+315) | GPIIb, vWF, PF4, Endothelin-1, VLA-4, PECAM-1 | 35 |
| NFκB | GGG(A/G)N2(C/T)(C/T)CC | GGGGCTCCC GGGAGCTCCC | (−218) D/R (+27) D/R | E-selectin, VCAM-1, ICAM-1, PECAM-1 | 36 |
| Sp1 | (T/G)(A/G)GGC(T/G)(A/G)(A/G)(T/G) | ACCAGCCT CCCAGCCCC CCCCGCCCA CCAGCCCA CCCAGCCCC | (−194) R (−37) R (+124) R (+150) R (+154) R | GPIIb, vWF, PF4, Endothelin-1, P-selectin, E-selectin, ICAM-1 | 37 |
Abbreviations: GPIIb, integrin subunit β3; vWF, von Willebrand factor; PF4, platelet factor 4; VCAM-1, vascular cellular adhesion molecule 1; ICAM-1, intercellular adhesion molecule 1; VLA-4, integrin subunit α4; PECAM-1, platelet-endothelial cell adhesion molecule 1.
The putative consensus sequences were obtained by submitting the endoglin promoter sequence to an Internet search at the EMBL data bank and consulting previous studies.38 39