Putative TGF-β and Steroid Responsive Elements Found on the Endoglin Promoter
| Response Element . | Consensus Sequence . | Endoglin Sequence (position)* . | Reference . | |
|---|---|---|---|---|
| Mad | GCCGNCGC | GCCGTCGC | (+225) | 40 |
| CGCCGGC | (+272) R | |||
| TAE | TCGN5GCCAAG | CTTTAGCCAAG) | (−285) | 41 |
| TGGCACTTCC | (−72) R | |||
| TTGGCAGGCG | (−53) R | |||
| CGGCCTGGCC | (−45) | |||
| TGGCCCAGCC | (−40) R | |||
| GTCACTGCCA | (+91) | |||
| TCGCTCCGGCC | (+229) | |||
| CGTGCGAGCC | (+260) | |||
| CGCACAGGCC | (+327) | |||
| GGCCCCCACG | (+333) R | |||
| TCE | GCGTGGGGA | GTGGGG | (−220) | 42 |
| GgGTGGGG | (−167) | |||
| GCtTGGGGA | (−158) | |||
| CCCACGC | (+268) R | |||
| CCCCACG | (+336) R | |||
| GgGTGGGGA | (+437) | |||
| TIE | GN2TTGGTGA | TagCCAAGCC | (−282) R | 43 |
| GCCTTGGaGA | (−190) | |||
| ERE | AGGTCAN3TGACCT | GGTCACAA | (−308) D/R | 44 |
| GGTCATAC | (+44) D/R | |||
| TCCTGACC | (+182) D/R | |||
| GRE | GGTACAN3TGTTCT | ACAGCAT | (+346) D/R | 44 |
| VDRE | (A/G)GGT(G/C)AN2(A/G)(A/G)GGNCA | GGGTCACAAA GCCCCTTCTC CTCCTGACCC GAGCAGGGAC GGCCCCGGTG | (−309) (−33) R (+181) R (+215) (+276) R | 45 |
| Response Element . | Consensus Sequence . | Endoglin Sequence (position)* . | Reference . | |
|---|---|---|---|---|
| Mad | GCCGNCGC | GCCGTCGC | (+225) | 40 |
| CGCCGGC | (+272) R | |||
| TAE | TCGN5GCCAAG | CTTTAGCCAAG) | (−285) | 41 |
| TGGCACTTCC | (−72) R | |||
| TTGGCAGGCG | (−53) R | |||
| CGGCCTGGCC | (−45) | |||
| TGGCCCAGCC | (−40) R | |||
| GTCACTGCCA | (+91) | |||
| TCGCTCCGGCC | (+229) | |||
| CGTGCGAGCC | (+260) | |||
| CGCACAGGCC | (+327) | |||
| GGCCCCCACG | (+333) R | |||
| TCE | GCGTGGGGA | GTGGGG | (−220) | 42 |
| GgGTGGGG | (−167) | |||
| GCtTGGGGA | (−158) | |||
| CCCACGC | (+268) R | |||
| CCCCACG | (+336) R | |||
| GgGTGGGGA | (+437) | |||
| TIE | GN2TTGGTGA | TagCCAAGCC | (−282) R | 43 |
| GCCTTGGaGA | (−190) | |||
| ERE | AGGTCAN3TGACCT | GGTCACAA | (−308) D/R | 44 |
| GGTCATAC | (+44) D/R | |||
| TCCTGACC | (+182) D/R | |||
| GRE | GGTACAN3TGTTCT | ACAGCAT | (+346) D/R | 44 |
| VDRE | (A/G)GGT(G/C)AN2(A/G)(A/G)GGNCA | GGGTCACAAA GCCCCTTCTC CTCCTGACCC GAGCAGGGAC GGCCCCGGTG | (−309) (−33) R (+181) R (+215) (+276) R | 45 |
Abbreviations: Mad, mothers against decapentaplegic complex; TAE, TGF-β activating element; TCE, TGF-β control element; TIE, TGF-β inhibitory element; ERE, estrogen response element; GRE, glucocorticoid response element; VDRE, vitamin D response element.
The putative consensus sequences were obtained by submitting the endoglin promoter sequence to an Internet search at the EMBL data bank and consulting previous studies.38 39