Expression of NK1.1, MAC-3, and CD3 in the Spleen After IL-2 Stimulation
| Strain . | Medium . | IL-2 . | ||||
|---|---|---|---|---|---|---|
| CD3 . | NK1.1 . | MAC-3 . | CD3 . | NK1.1 . | MAC-3 . | |
| Wild-type | 76.85 ± 2.85 | 14.75 ± 2.25 | 0.2 ± 0 | 79.35 ± 6.35 | 11.75 ± 3.25 | 1.5 ± 1.2 |
| PD | 83.7 ± 10.8 | 9.05 ± 0.15 | 0.9 ± 0.5 | 85.15 ± 5.05 | 6.1 ± 2.1 | 0.65 ± 0.35 |
| gld | 83.15 ± 8.05 | 13.9 ± 4.3 | 0.75 ± 0.35 | 85.85 ± 2.35 | 15.2 ± 1.1 | 0.35 ± 0.25 |
| Strain . | Medium . | IL-2 . | ||||
|---|---|---|---|---|---|---|
| CD3 . | NK1.1 . | MAC-3 . | CD3 . | NK1.1 . | MAC-3 . | |
| Wild-type | 76.85 ± 2.85 | 14.75 ± 2.25 | 0.2 ± 0 | 79.35 ± 6.35 | 11.75 ± 3.25 | 1.5 ± 1.2 |
| PD | 83.7 ± 10.8 | 9.05 ± 0.15 | 0.9 ± 0.5 | 85.15 ± 5.05 | 6.1 ± 2.1 | 0.65 ± 0.35 |
| gld | 83.15 ± 8.05 | 13.9 ± 4.3 | 0.75 ± 0.35 | 85.85 ± 2.35 | 15.2 ± 1.1 | 0.35 ± 0.25 |
Splenic nylon wool purified T cells from wild-type, PD, andgld mice were cultured with medium or IL-2 and stained for NK1.1, MAC-3, and CD3 markers and analyzed flow cytometrically. The data are depicted as mean percent positive cells ± SEM. Data are representative of three experiments. There was no statistically significant difference between various groups.
Abbreviation: PD, perforin-deficient.