Properties of Cell Lines Expressing Mutant Human GM-CSF Receptors
| Cell Line . | GM-CSF Receptor Content . | kd (×10−11 mol/L) . | High- Affinity Receptors/ Cell . | Protein Synthesis IC50 (×10−12mol/L) . | Proliferation IC50 (×10−12mol/L) . |
|---|---|---|---|---|---|
| M1α2 | α alone | ND | 0 | >4,000 | >4,000 |
| M1β7 | β alone | ND | 0 | >4,000 | >4,000 |
| M1β7T1 | β plus term1 α | 4.5 | 2,700 | 10 | 10 |
| M1β7α2 | β plus α | 13 | 9,400 | 4 | 2 |
| 32Dα2 | α | ND | 0 | 120 | 80 |
| 32Dβ2 | β | ND | 0 | >4,000 | >4,000 |
| 32Dβ2T1 | β plus term1 α | 2 | 4,100 | 10 | 400 |
| 32Dβ2α2 | β plus α | 3 | 3,300 | 1 | 4 |
| Cell Line . | GM-CSF Receptor Content . | kd (×10−11 mol/L) . | High- Affinity Receptors/ Cell . | Protein Synthesis IC50 (×10−12mol/L) . | Proliferation IC50 (×10−12mol/L) . |
|---|---|---|---|---|---|
| M1α2 | α alone | ND | 0 | >4,000 | >4,000 |
| M1β7 | β alone | ND | 0 | >4,000 | >4,000 |
| M1β7T1 | β plus term1 α | 4.5 | 2,700 | 10 | 10 |
| M1β7α2 | β plus α | 13 | 9,400 | 4 | 2 |
| 32Dα2 | α | ND | 0 | 120 | 80 |
| 32Dβ2 | β | ND | 0 | >4,000 | >4,000 |
| 32Dβ2T1 | β plus term1 α | 2 | 4,100 | 10 | 400 |
| 32Dβ2α2 | β plus α | 3 | 3,300 | 1 | 4 |
M1β7 are M1 cells expressing the wild-type human GM-CSF receptor β chain.
Abbreviations: T1, term1 mutant human GM-CSF receptor α chain; α2, human GM-CSF receptor α chain; 32Dβ2, 32D cells expressing the wild-type human GM-CSF receptor β chain; M1α2, M1 cells expressing α2 α chain.