The Effects of CD48 MoAb Infusion on Splenic Lymphohematopoietic Reconstitution in Lethally Irradiated Recipients of Congenic BM Grafts
| Tissue . | Day . | αCD48 . | Total No. . | Donor . | CD4+ . | CD8+ . | CD48+ . | B220+ . | Mac1+ . | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor . | Host . | Donor . | Host . | ||||||||
| Spleen | 7 | No | 13.5 (5.0) | 5.2 (0.7) | 0.0 (0.0) | 0.5 (0.0) | 0.1 (0.0) | 0.1 (0.0) | 5.7 (0.6) | NE | NE |
| Spleen | 7 | Yes | 2.2 (0.4)5-152 | 0.9 (0.0)5-151 | 0.0 (0.0) | 0.3 (0.0)5-151 | 0.0 (0.0)5-151 | 0.0 (0.0)5-151 | 0.1 (0.0)5-151 | NE | NE |
| Spleen | 10 | No | 14.9 (2.4) | 11.1 (1.5) | 0.3 (0.1) | 0.2 (0.1) | 0.3 (0.1) | 0.0 (0.0) | 8.2 (1.6) | 3.3 (0.8) | 7.3 (1.2) |
| Spleen | 10 | Yes | 17.3 (4.4) | 6.3 (0.9)5-150 | 0.3 (0.2) | 0.3 (0.1) | 0.4 (0.2) | 0.0 (0.0) | 0.5 (0.1)5-151 | 1.7 (0.5) | 3.9 (0.5)5-152 |
| Spleen | 14 | No | 22.2 (3.6) | 19.5 (3.6) | 0.6 (0.2) | 0.3 (0.1) | 0.7 (0.3) | 0.1 (0.1) | 15.3 (3.5) | 8.7 (3.0) | 8.2 (0.3) |
| Spleen | 14 | Yes | 12.8 (5.7) | 10.9 (4.8) | 0.5 (0.3) | 0.1 (0.1) | 0.2 (0.1) | 0.0 (0.0) | 0.2 (0.1)5-151 | 2.1 (0.5)5-152 | 6.6 (0.5) |
| Spleen | 20 | No | 68.8 (19.1) | 65.0 (18.7) | 2.3 (0.8) | 1.3 (0.4) | 1.7 (0.4) | 0.4 (0.2) | 61.2 (20.0) | 45.4 (18.6) | 14.2 (2.4) |
| Spleen | 20 | Yes | 29.5 (15.2) | 26.1 (13.0) | 1.2 (0.9) | 1.6 (0.9) | 1.3 (1.1) | 0.7 (0.4) | 1.7 (1.2)5-151 | 11.6 (3.7) | 9.7 (8.3) |
| Tissue . | Day . | αCD48 . | Total No. . | Donor . | CD4+ . | CD8+ . | CD48+ . | B220+ . | Mac1+ . | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor . | Host . | Donor . | Host . | ||||||||
| Spleen | 7 | No | 13.5 (5.0) | 5.2 (0.7) | 0.0 (0.0) | 0.5 (0.0) | 0.1 (0.0) | 0.1 (0.0) | 5.7 (0.6) | NE | NE |
| Spleen | 7 | Yes | 2.2 (0.4)5-152 | 0.9 (0.0)5-151 | 0.0 (0.0) | 0.3 (0.0)5-151 | 0.0 (0.0)5-151 | 0.0 (0.0)5-151 | 0.1 (0.0)5-151 | NE | NE |
| Spleen | 10 | No | 14.9 (2.4) | 11.1 (1.5) | 0.3 (0.1) | 0.2 (0.1) | 0.3 (0.1) | 0.0 (0.0) | 8.2 (1.6) | 3.3 (0.8) | 7.3 (1.2) |
| Spleen | 10 | Yes | 17.3 (4.4) | 6.3 (0.9)5-150 | 0.3 (0.2) | 0.3 (0.1) | 0.4 (0.2) | 0.0 (0.0) | 0.5 (0.1)5-151 | 1.7 (0.5) | 3.9 (0.5)5-152 |
| Spleen | 14 | No | 22.2 (3.6) | 19.5 (3.6) | 0.6 (0.2) | 0.3 (0.1) | 0.7 (0.3) | 0.1 (0.1) | 15.3 (3.5) | 8.7 (3.0) | 8.2 (0.3) |
| Spleen | 14 | Yes | 12.8 (5.7) | 10.9 (4.8) | 0.5 (0.3) | 0.1 (0.1) | 0.2 (0.1) | 0.0 (0.0) | 0.2 (0.1)5-151 | 2.1 (0.5)5-152 | 6.6 (0.5) |
| Spleen | 20 | No | 68.8 (19.1) | 65.0 (18.7) | 2.3 (0.8) | 1.3 (0.4) | 1.7 (0.4) | 0.4 (0.2) | 61.2 (20.0) | 45.4 (18.6) | 14.2 (2.4) |
| Spleen | 20 | Yes | 29.5 (15.2) | 26.1 (13.0) | 1.2 (0.9) | 1.6 (0.9) | 1.3 (1.1) | 0.7 (0.4) | 1.7 (1.2)5-151 | 11.6 (3.7) | 9.7 (8.3) |
B6-CD45.1 mice were lethally irradiated and administered congenic B6-CD45.2 BM as described in Materials and Methods. Beginning on days −1 and +2 and continuing twice weekly through day +14 post-BMT, mice were administered irrelevant IgG or αCD48 MoAb (300 μg/dose) IP. At the indicated times post-BMT, three mice per group were individually analyzed for the absolute number of cells present in the spleen compartment. Flow cytometric analysis was performed to determine the absolute number of donor (CD45.2+) cells, CD4+, CD8+, B220+, Mac1+, and CD48+ cells present in each compartment. A representative example of the flow cytometry for CD48 expression is shown in Fig 3B. Values expressed at the mean values × 10−6. The numbers in parenthesis are 1 SEM.
Abbreviation: NE, not evaluable.
P < .05 as compared with irrelevant IgG controls.
P < .01 as compared with irrelevant IgG controls.
0.1 > P > .05 as compared with irrelevant IgG controls.