Table 6. Effect of CD48 MoAb Infusion... | American Society of Hematology

Table 6.

Effect of CD48 MoAb Infusion on Lymphohematopoiesis in the BM and Spleen of Non-BMT Controls

Group	Total No.	% CD4⁺	% CD8⁺	% CD19⁺	% Mac1⁺	CD48
Group	Total No.	% CD4⁺	% CD8⁺	% CD19⁺	% Mac1⁺	Neg	Lo	Med	Hi
BM
Irrelevant MoAb	41 ± 1	1 ± 0	1 ± 0	19 ± 1	54 ± 2	7 ± 0	52 ± 1	28 ± 1	13 ± 1
αCD48 MoAb	37 ± 2	1 ± 0	1 ± 0	21 ± 4	48 ± 5	72 ± 2^6-150	27 ± 2^6-150	0 ± 0^6-150	0 ± 0^6-150

Group	Total No.	% CD4⁺	% CD8⁺	% CD19⁺	% Mac1⁺	CD48
Group	Total No.	% CD4⁺	% CD8⁺	% CD19⁺	% Mac1⁺	Neg	Lo	Med	Hi
BM
Irrelevant MoAb	41 ± 1	1 ± 0	1 ± 0	19 ± 1	54 ± 2	7 ± 0	52 ± 1	28 ± 1	13 ± 1
αCD48 MoAb	37 ± 2	1 ± 0	1 ± 0	21 ± 4	48 ± 5	72 ± 2^6-150	27 ± 2^6-150	0 ± 0^6-150	0 ± 0^6-150

Group	Total No.	% CD4⁺	% CD8⁺	% CD19⁺	% Mac1⁺	CD48
Group	Total No.	% CD4⁺	% CD8⁺	% CD19⁺	% Mac1⁺	Neg	Pos
Spleen
Irrelevant MoAb	58 ± 1	18 ± 0	9 ± 1	50 ± 1	6 ± 1	1 ± 0	99 ± 0
αCD48 MoAb	62 ± 9	21 ± 0	8 ± 1	54 ± 1	6 ± 2	92 ± 1^6-150	8 ± 0^6-150

Group	Total No.	% CD4⁺	% CD8⁺	% CD19⁺	% Mac1⁺	CD48
Group	Total No.	% CD4⁺	% CD8⁺	% CD19⁺	% Mac1⁺	Neg	Pos
Spleen
Irrelevant MoAb	58 ± 1	18 ± 0	9 ± 1	50 ± 1	6 ± 1	1 ± 0	99 ± 0
αCD48 MoAb	62 ± 9	21 ± 0	8 ± 1	54 ± 1	6 ± 2	92 ± 1^6-150	8 ± 0^6-150

B6 mice were administered irrelevant IgG or αCD48 MoAb (300 μg/dose) IP on days 0, 3, and 7. On day 9, three mice per group were individually analyzed for the absolute number of cells present in the BM and spleen compartments. Flow cytometric analysis was performed to determine the absolute number of CD4⁺, CD8⁺, B220⁺, Mac1⁺, and CD48⁺ cells (listed according to the level of surface expression) present in each compartment. A representative example of the flow cytometry for CD48 expression is shown in Fig 3. Values expressed are the mean values × 10⁻⁶ ± 1 SEM.

F6-150

P ≤ .05 as compared with irrelevant MoAb-treated controls.

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