Modulation of CD116 (GM-CSFR) and CD115 Expression by IL-6 and M-CSF
| Cuture Condition . | Antibodies . | CD116 Expression . | CD115 Expression . | ||
|---|---|---|---|---|---|
| % . | MFI . | % . | MFI . | ||
| Medium | 31.5 | 45.8 | 43.3 | 108 | |
| CLB-CHA CM | Control antibodies | 3.3 | 32.5 | 89.9 | 305 |
| Anti–IL-6 + IL-6R | 2.3 | 37.1 | 77.6 | 165 | |
| Anti–M-CSF | 21.9 | 38.2 | 62.7 | 106 | |
| Anti–IL-6 + IL-6R + Anti–M-CSF | 23.7 | 45.1 | 44.3 | 86 | |
| CLB-VER CM | Control antibodies | 4.7 | 30.5 | 83.1 | 232 |
| Anti–IL-6 + IL-6R | 5.9 | 35.1 | 71.7 | 153 | |
| Anti–M-CSF | 18.7 | 36.7 | 40.5 | 92 | |
| Anti–IL-6 + IL-6R + anti–M-CSF | 25.8 | 42.8 | 41.7 | 81 | |
| IL-6 (20 ng/mL) | 15.9 | 37.9 | 79.6 | 309 | |
| M-CSF (20 ng/mL) | 3.3 | 35.6 | 68.0 | 156 | |
| IL-6 + M-CSF (20 ng/mL) | 3.3 | 31.9 | 86.6 | 291 | |
| Cuture Condition . | Antibodies . | CD116 Expression . | CD115 Expression . | ||
|---|---|---|---|---|---|
| % . | MFI . | % . | MFI . | ||
| Medium | 31.5 | 45.8 | 43.3 | 108 | |
| CLB-CHA CM | Control antibodies | 3.3 | 32.5 | 89.9 | 305 |
| Anti–IL-6 + IL-6R | 2.3 | 37.1 | 77.6 | 165 | |
| Anti–M-CSF | 21.9 | 38.2 | 62.7 | 106 | |
| Anti–IL-6 + IL-6R + Anti–M-CSF | 23.7 | 45.1 | 44.3 | 86 | |
| CLB-VER CM | Control antibodies | 4.7 | 30.5 | 83.1 | 232 |
| Anti–IL-6 + IL-6R | 5.9 | 35.1 | 71.7 | 153 | |
| Anti–M-CSF | 18.7 | 36.7 | 40.5 | 92 | |
| Anti–IL-6 + IL-6R + anti–M-CSF | 25.8 | 42.8 | 41.7 | 81 | |
| IL-6 (20 ng/mL) | 15.9 | 37.9 | 79.6 | 309 | |
| M-CSF (20 ng/mL) | 3.3 | 35.6 | 68.0 | 156 | |
| IL-6 + M-CSF (20 ng/mL) | 3.3 | 31.9 | 86.6 | 291 | |
CD34+ cells were cultured in presence of GM-CSF + TNFα alone (medium), with 10% RCC CM (CLB-VER, CLB-CHA), or in presence of recombinant cytokines (IL-6, M-CSF, IL-6 + M-CSF; 20 ng/mL) from day 6 to 12. Control antibodies (MoAb and polyclonal controls; 10 μg/mL) or antibodies against IL-6 + IL-6R (10 μg/mL) and M-CSF (10 μg/mL) were added alone or in combination. At day 12, cells were harvested and processed for staining with mouse and rat control antibodies (not shown), CD116-PE (GM-CSFRα), or CD115 (M-CSF-R) + antirat (IgG1)-PE. Results are expressed as the percentage and MFI of positive cells. Results are representative of three different experiments.