Flow Cytometric Analysis of K562/B3 Clone 1
| Antibody . | Directed Against . | Fluorescence Intensity for K562/pBp (day 1) . | Fluorescence Intensity for K562/B3 Clone 1 (day 1) . | Mean (±SD) Ratio of Fluorescence Intensity for K562/B3 Clone 1 over K562/pBp (measured daily over 3 consecutive days) . |
|---|---|---|---|---|
| BRIC 169 | B3 (intracellular) | 3.0 | 4.0 | 1.4 ± 0.1 |
| BRIC 6 | B3 (extracellular) | 3.9 | 40 | 9.9 ± 0.7 |
| BRIC 14 | Wrb | 4.3 | 206 | 42 ± 5.1 |
| BRIC 201 | Wrb | 2.8 | 15 | 8.1 ± 2.2 |
| R10 | Glycophorin A | 117 | 259 | 2.5 ± 0.3 |
| R18 | Glycophorin A | 123 | 266 | 2.7 ± 0.6 |
| BRIC 4 | Glycophorin C | 144 | 152 | 1.2 ± 0.1 |
| BRIC 69 | Rh polypeptides | 5.7 | 18 | 3.0 ± 0.2 |
| LA 18.18 | Rh glycoprotein | 38 | 61 | 2.2 ± 0.6 |
| BS46 | LW | 5.6 | 10 | 2.1 ± 0.2 |
| BRIC 126 | CD 47 | 34 | 41 | 1.5 ± 0.3 |
| CBC-512 | Fy3 | 2.8 | 3.7 | 1.4 ± 0.1 |
| Antibody . | Directed Against . | Fluorescence Intensity for K562/pBp (day 1) . | Fluorescence Intensity for K562/B3 Clone 1 (day 1) . | Mean (±SD) Ratio of Fluorescence Intensity for K562/B3 Clone 1 over K562/pBp (measured daily over 3 consecutive days) . |
|---|---|---|---|---|
| BRIC 169 | B3 (intracellular) | 3.0 | 4.0 | 1.4 ± 0.1 |
| BRIC 6 | B3 (extracellular) | 3.9 | 40 | 9.9 ± 0.7 |
| BRIC 14 | Wrb | 4.3 | 206 | 42 ± 5.1 |
| BRIC 201 | Wrb | 2.8 | 15 | 8.1 ± 2.2 |
| R10 | Glycophorin A | 117 | 259 | 2.5 ± 0.3 |
| R18 | Glycophorin A | 123 | 266 | 2.7 ± 0.6 |
| BRIC 4 | Glycophorin C | 144 | 152 | 1.2 ± 0.1 |
| BRIC 69 | Rh polypeptides | 5.7 | 18 | 3.0 ± 0.2 |
| LA 18.18 | Rh glycoprotein | 38 | 61 | 2.2 ± 0.6 |
| BS46 | LW | 5.6 | 10 | 2.1 ± 0.2 |
| BRIC 126 | CD 47 | 34 | 41 | 1.5 ± 0.3 |
| CBC-512 | Fy3 | 2.8 | 3.7 | 1.4 ± 0.1 |
Abbreviation: B3, band 3.