Flow Cytometric Analysis of K562/RhD + B3 and K562/RhcE [6e0d] + B3 Clones
| Antibody . | Directed Against . | Mean Fluorescence Intensity (±SD) for 12 K562/RhD + pBn Clones . | Fluorescence Intensity of K562/RhD + B3 Clone 1 . | Fluorescence Intensity of K562/RhD + B3 Clone 2 . | Fluorescence Intensity of K562/RhD + B3 Clone 3 . |
|---|---|---|---|---|---|
| TCM (i) | 2.7 ± 0.2 | 3.2 | 3.7 | 2.8 | |
| BRIC 6 | Band 3 | 2.8 ± 0.2 | 33 | 17 | 12 |
| BRIC 69 | Rh30 | 19 ± 5.3 | 69 | 54 | 37 |
| LA18.18 | RhGP | 19 ± 3.6 | 113 | 122 | 88 |
| TCM (ii) | 2.8 ± 0.2 | 3.4 | 3.6 | 2.6 | |
| Brad 5 | RhD | 23 ± 6.6 | 45 | 36 | 32 |
| Mean Fluorescence Intensity (±SD) for 11 K562/RhcE + pBn Clones | Fluorescence Intensity of K562/RhcE + B3 Clone 1 | Fluorescence Intensity of K562/RhcE + B3 Clone 2 | Fluorescence Intensity of K562/RhcE + B3 Clone 3 | ||
| TCM (i) | 3.5 ± 0.6 | 3.4 | 3.4 | 3.6 | |
| BRIC 6 | Band 3 | 4.0 ± 0.7 | 42 | 14 | 13 |
| BRIC 69 | Rh30 | 24 ± 8.1 | 140 | 107 | 107 |
| LA18.18 | RhGP | 24 ± 7.4 | 211 | 208 | 212 |
| TCM (ii) | 3.6 ± 0.4 | 3.9 | 4.1 | 4.3 | |
| MS37 | Rhc | 10 ± 2.6 | 30 | 34 | 29 |
| Antibody . | Directed Against . | Mean Fluorescence Intensity (±SD) for 12 K562/RhD + pBn Clones . | Fluorescence Intensity of K562/RhD + B3 Clone 1 . | Fluorescence Intensity of K562/RhD + B3 Clone 2 . | Fluorescence Intensity of K562/RhD + B3 Clone 3 . |
|---|---|---|---|---|---|
| TCM (i) | 2.7 ± 0.2 | 3.2 | 3.7 | 2.8 | |
| BRIC 6 | Band 3 | 2.8 ± 0.2 | 33 | 17 | 12 |
| BRIC 69 | Rh30 | 19 ± 5.3 | 69 | 54 | 37 |
| LA18.18 | RhGP | 19 ± 3.6 | 113 | 122 | 88 |
| TCM (ii) | 2.8 ± 0.2 | 3.4 | 3.6 | 2.6 | |
| Brad 5 | RhD | 23 ± 6.6 | 45 | 36 | 32 |
| Mean Fluorescence Intensity (±SD) for 11 K562/RhcE + pBn Clones | Fluorescence Intensity of K562/RhcE + B3 Clone 1 | Fluorescence Intensity of K562/RhcE + B3 Clone 2 | Fluorescence Intensity of K562/RhcE + B3 Clone 3 | ||
| TCM (i) | 3.5 ± 0.6 | 3.4 | 3.4 | 3.6 | |
| BRIC 6 | Band 3 | 4.0 ± 0.7 | 42 | 14 | 13 |
| BRIC 69 | Rh30 | 24 ± 8.1 | 140 | 107 | 107 |
| LA18.18 | RhGP | 24 ± 7.4 | 211 | 208 | 212 |
| TCM (ii) | 3.6 ± 0.4 | 3.9 | 4.1 | 4.3 | |
| MS37 | Rhc | 10 ± 2.6 | 30 | 34 | 29 |
Abbreviations: TCM (i), negative control, incubating cells first in tissue culture medium, and then in rabbit anti-mouse/FITC; TCM (ii), negative control, incubating cells first in tissue culture medium, and then in rabbit anti-human/FITC; Rh30, Rh polypeptides; RhGP, Rh glycoprotein.