Stimulatory Capacity of Mononuclear Cells Cultured With Various Cytokine Combinations
| Cytokines* . | Allogeneic MLR† . |
|---|---|
| Flt3L/IL-4 | 20,341 ± 1,815 |
| Flt3L/IL-4/TNF-α | 32,387 ± 2,610 |
| Flt3L/IL-4/CD40L | 37,532 ± 2,815 |
| CD40L | 28,419 ± 3,301 |
| CD40L/IL-4 | 33,080 ± 5,528 |
| CD40L/IL-4/GM-CSF | 44,130 ± 3,421 |
| GM-CSF/IL-4/TNF-α | 38,761 ± 957 |
| GM-CSF/IL-4 | 21,213 ± 2,030 |
| PBMNC | 6,349 ± 259 |
| Medium | 484 ± 64 |
| Cytokines* . | Allogeneic MLR† . |
|---|---|
| Flt3L/IL-4 | 20,341 ± 1,815 |
| Flt3L/IL-4/TNF-α | 32,387 ± 2,610 |
| Flt3L/IL-4/CD40L | 37,532 ± 2,815 |
| CD40L | 28,419 ± 3,301 |
| CD40L/IL-4 | 33,080 ± 5,528 |
| CD40L/IL-4/GM-CSF | 44,130 ± 3,421 |
| GM-CSF/IL-4/TNF-α | 38,761 ± 957 |
| GM-CSF/IL-4 | 21,213 ± 2,030 |
| PBMNC | 6,349 ± 259 |
| Medium | 484 ± 64 |
Adherent PBMNC were cultured with the indicated combinations of cytokines for 7 days.
105 responding cells from allogeneic PBMNC were cultured with 103 irradiated stimulator cells (DC or PBMNC). Thymidine incorporation was measured on day 5 by a 16-hour pulse with3H-thymidine. The assay was conducted in triplicate and results show the means ± SD in cpm from one representative experiment out of four.