Angiostatin Preparations Used in This Study
| Species . | Abbr. . | Kringles . | Amino Acid Residues* . | Source . | Reference . | LPS Levels ng/mg Protein† . |
|---|---|---|---|---|---|---|
| Human | ehAst | K1-K3 | Tyr80-Val338 Tyr80-Val354 | Elastase-digested plasminogen | 59 | 4.5-9.2 |
| Human | K1-K4 | Leu74-Leu451 | Recombinant P pastoris | 17 | 0.40 | |
| Human | rhAst | K1-K4 | Leu74-Leu451 | Recombinant CHO cells | MacDonald et al (in preparation) | 0.43 |
| Human | phAst | K1-K4 + 2/3 of K5 | Lys78-? | Plasmin autocatalysis | 31 | <0.001 |
| Mouse | rmAst | NTP + K1-K4 | Asp1-Ser13; Arg78-Gly439 | Recombinant baculovirus | 18 | 0.27-1.74 |
| Human | hPlg | Plasminogen | Plasma | 0.02-0.09 |
| Species . | Abbr. . | Kringles . | Amino Acid Residues* . | Source . | Reference . | LPS Levels ng/mg Protein† . |
|---|---|---|---|---|---|---|
| Human | ehAst | K1-K3 | Tyr80-Val338 Tyr80-Val354 | Elastase-digested plasminogen | 59 | 4.5-9.2 |
| Human | K1-K4 | Leu74-Leu451 | Recombinant P pastoris | 17 | 0.40 | |
| Human | rhAst | K1-K4 | Leu74-Leu451 | Recombinant CHO cells | MacDonald et al (in preparation) | 0.43 |
| Human | phAst | K1-K4 + 2/3 of K5 | Lys78-? | Plasmin autocatalysis | 31 | <0.001 |
| Mouse | rmAst | NTP + K1-K4 | Asp1-Ser13; Arg78-Gly439 | Recombinant baculovirus | 18 | 0.27-1.74 |
| Human | hPlg | Plasminogen | Plasma | 0.02-0.09 |
Abbreviations: Abbr., abbreviation used in this study; K, kringle; NTP, N-terminal peptide.
Numbering is based on published sequences of human (791 residues)60 and mouse (793 residues)61plasminogens and begins at Glu1 and Asp1 in human and mouse, respectively (ie, excluding the 19 amino acid signal peptide that begins at Met-19).
LPS levels were determined using the LAL assay.