T-cell Recovery After T-cell Targeting With CD3x19 bs ab in Vivo
| Injection of: . | T Cells Alone . | T Cells, B Cells . | T Cells, B Cells Plus Anti-CD3 and anti-CD19 . | T Cells, B Cells Plus Anti-CD3x19 . |
|---|---|---|---|---|
| Without anti-CD28 | 3.7 × 106 ± 0.25 × 106 | 3.4 × 106 ± 0.41 × 106 | 3.3 × 106 ± 0.21 × 106 | 3.8 × 106 ± 0.34 × 106 |
| With anti-CD28 | 3.5 × 106 ± 0.21 × 106 | 2.9 × 106 ± 0.6 × 106 | 3.1 × 106 ± 0.45 × 106 | 3.4 × 106 ± 0.3 × 106 |
| Injection of: . | T Cells Alone . | T Cells, B Cells . | T Cells, B Cells Plus Anti-CD3 and anti-CD19 . | T Cells, B Cells Plus Anti-CD3x19 . |
|---|---|---|---|---|
| Without anti-CD28 | 3.7 × 106 ± 0.25 × 106 | 3.4 × 106 ± 0.41 × 106 | 3.3 × 106 ± 0.21 × 106 | 3.8 × 106 ± 0.34 × 106 |
| With anti-CD28 | 3.5 × 106 ± 0.21 × 106 | 2.9 × 106 ± 0.6 × 106 | 3.1 × 106 ± 0.45 × 106 | 3.4 × 106 ± 0.3 × 106 |
107 T cells were injected intraperitoneally in the presence or absence of 107 irradiated B cells (Nalm-6) plus/minus antibodies. After 16 hours the cells were washed out and stained with CD2-FITC conjugate. Absolute numbers of CD2+cells are given as calculated from total cell numbers and the percentage of CD2+ cells after the peritoneal washout and immunomagnetic separation procedure, ie, the removal of the B cells via immunomagnetic B-cell depletion. Mean ± SD from three animals per group are shown.