The Effect of hG-CSF on Colony Formation From BM Cells
| Mice . | Cytokines . | No. of Colonies (2.5 × 104 BM cells) . | ||||||
|---|---|---|---|---|---|---|---|---|
| GM . | B . | Mk . | Mast . | Mix . | Blast . | Total . | ||
| Littermate | No factor | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| hG-CSF | 32 ± 5 | 0 | 0 | 0 | 0 | 0 | 32 ± 5 | |
| EPO | 0 | 2 ± 1 | 0 | 0 | 0 | 0 | 2 ± 2 | |
| hG-CSF + EPO | 34 ± 6 | 2 ± 1 | 0 | 0 | 0 | 0 | 36 ± 6 | |
| Tg Mouse | No factor | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| hG-CSF | 66 ± 9 | 0 | 5 ± 2 | 4 ± 2 | 4 ± 2 | 4 ± 2 | 83 ± 8 | |
| EPO | 0 | 2 ± 1 | 0 | 0 | 0 | 0 | 2 ± 1 | |
| hG-CSF + EPO | 66 ± 10 | 6 ± 2 | 7 ± 2 | 4 ± 2 | 5 ± 2 | 3 ± 1 | 91 ± 10 | |
| Mice . | Cytokines . | No. of Colonies (2.5 × 104 BM cells) . | ||||||
|---|---|---|---|---|---|---|---|---|
| GM . | B . | Mk . | Mast . | Mix . | Blast . | Total . | ||
| Littermate | No factor | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| hG-CSF | 32 ± 5 | 0 | 0 | 0 | 0 | 0 | 32 ± 5 | |
| EPO | 0 | 2 ± 1 | 0 | 0 | 0 | 0 | 2 ± 2 | |
| hG-CSF + EPO | 34 ± 6 | 2 ± 1 | 0 | 0 | 0 | 0 | 36 ± 6 | |
| Tg Mouse | No factor | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| hG-CSF | 66 ± 9 | 0 | 5 ± 2 | 4 ± 2 | 4 ± 2 | 4 ± 2 | 83 ± 8 | |
| EPO | 0 | 2 ± 1 | 0 | 0 | 0 | 0 | 2 ± 1 | |
| hG-CSF + EPO | 66 ± 10 | 6 ± 2 | 7 ± 2 | 4 ± 2 | 5 ± 2 | 3 ± 1 | 91 ± 10 | |
BM cells (2.5 × 104) were incubated in the presence of hG-CSF (20 ng/mL) and/or EPO (2 U/mL). Values are the mean ± SD of three independent experiments.
Abbreviations: GM, granulocyte-macrophage colonies; B, burst-forming unit-erythroid; Mk, megakaryocyte colonies; Mast, mast cell colonies; Mix, mixed hematopoietic colonies.