The Effect of hG-CSF on the Number of CFU-S Progenitors In Vivo
| Mice . | Injection . | CFU-S Per Femur . | CFU-S Per Spleen . | ||
|---|---|---|---|---|---|
| Day 8 . | Day 12 . | Day 8 . | Day 12 . | ||
| Experiment no. 1 | |||||
| Littermates | PBS | 837 ± 218 | 837 ± 218 | 423 ± 110 | 680 ± 220 |
| hG-CSF | 567 ± 252 | 693 ± 126 | 3,191 ± 709 | 4,373 ± 709 | |
| Tg mice | PBS | 828 ± 216 | 828 ± 216 | 690 ± 230 | 759 ± 138 |
| hG-CSF | 2,363 ± 163 | 1,820 ± 163 | 13,018 ± 650 | 15,187 ± 650 | |
| Experiment no. 2 | |||||
| Littermates | PBS | 860 ± 116 | 720 ± 120 | 921 ± 196 | 921 ± 114 |
| hG-CSF | 430 ± 123 | 369 ± 123 | 4,600 ± 690 | 2,300 ± 230 | |
| Tg mice | PBS | 872 ± 108 | 681 ± 108 | 1,260 ± 240 | 740 ± 114 |
| hG-CSF | 1,980 ± 180 | 1,969 ± 103 | 16,200 ± 1,620 | 11,340 ± 1,080 | |
| Mice . | Injection . | CFU-S Per Femur . | CFU-S Per Spleen . | ||
|---|---|---|---|---|---|
| Day 8 . | Day 12 . | Day 8 . | Day 12 . | ||
| Experiment no. 1 | |||||
| Littermates | PBS | 837 ± 218 | 837 ± 218 | 423 ± 110 | 680 ± 220 |
| hG-CSF | 567 ± 252 | 693 ± 126 | 3,191 ± 709 | 4,373 ± 709 | |
| Tg mice | PBS | 828 ± 216 | 828 ± 216 | 690 ± 230 | 759 ± 138 |
| hG-CSF | 2,363 ± 163 | 1,820 ± 163 | 13,018 ± 650 | 15,187 ± 650 | |
| Experiment no. 2 | |||||
| Littermates | PBS | 860 ± 116 | 720 ± 120 | 921 ± 196 | 921 ± 114 |
| hG-CSF | 430 ± 123 | 369 ± 123 | 4,600 ± 690 | 2,300 ± 230 | |
| Tg mice | PBS | 872 ± 108 | 681 ± 108 | 1,260 ± 240 | 740 ± 114 |
| hG-CSF | 1,980 ± 180 | 1,969 ± 103 | 16,200 ± 1,620 | 11,340 ± 1,080 | |
The assay for CFU-S was performed using a modification of the method described in Materials and Methods. After hG-CSF or PBS administration for 7 consecutive days, the total number of CFU-S developed from BM cells per femur and spleen cells per mouse was estimated at 8 and 12 days. The two separate experiments are shown. Each mean ± SD was calculated from the data obtained from three or four mice.