Growth of MK Colonies From ET and PMF CD34+ Cells
| . | 0 . | PEG-rhuMGDF . | sMpl-Fc . | PEG-rhuMGDF + sMpl-Fc . |
|---|---|---|---|---|
| t1 | 7 ± 0.8 | 19 ± 1 | 0.6 ± 0.9 | 15 ± 1 |
| t6 | 8 ± 0 | 37 ± 4 | 1 ± 1 | 18 ± 2 |
| t7 | 9 | 43 | 2 | 28 |
| t8 | 1.6 ± 1 | 16 ± 9 | ND | ND |
| t9 | 6 ± 1 | 38 ± 1 | ND | ND |
| t12 | 18 ± 5 | 59 ± 10 | ND | ND |
| m1 | 15 ± 2 | 57 ± 6 | 0.3 ± 0.5 | 24 ± 6 |
| . | 0 . | PEG-rhuMGDF . | sMpl-Fc . | PEG-rhuMGDF + sMpl-Fc . |
|---|---|---|---|---|
| t1 | 7 ± 0.8 | 19 ± 1 | 0.6 ± 0.9 | 15 ± 1 |
| t6 | 8 ± 0 | 37 ± 4 | 1 ± 1 | 18 ± 2 |
| t7 | 9 | 43 | 2 | 28 |
| t8 | 1.6 ± 1 | 16 ± 9 | ND | ND |
| t9 | 6 ± 1 | 38 ± 1 | ND | ND |
| t12 | 18 ± 5 | 59 ± 10 | ND | ND |
| m1 | 15 ± 2 | 57 ± 6 | 0.3 ± 0.5 | 24 ± 6 |
Mean number of MK colonies ± SEM (revealed by immunocytochemistry) after 10 days of culture in serum-free fibrin-clot conditions, without growth factor (0) with PEG-rhuMGDF 10 ng/mL, with the soluble Mpl receptor at 5 μg/mL (sMpl-Fc), or with a combination of sMpl-Fc (5 μg/mL) and PEG-rhu MGDF (PEG-rhuMGDF + sMpl-Fc). Results are expressed for 1 × 103 CD34+ cells of TE (t) or PMF (m) patients.