Table 1. Analysis of GATA-2irGFP and... | American Society of Hematology

Table 1.

Analysis of GATA-2irGFP and Control irGFP Vector Expression in Bone Marrow Sca-1⁺ LIN⁻ Cells of Transplanted Mice

Mouse	Total Sca⁺ Lin⁻ Cells^‡ (×10⁵)	% GFP ⁺	Total GFP⁺ Sca⁺ Lin⁻ Cells (×10³)
irGFP 1^*	1.08	75	81
irGFP 2^*	1.20	84	101
irGFP 3^†	2.20	85	191
GATA-2irGFP 1^*	0.17	14	2.38
GATA-2irGFP 6^*	0.16	24	3.84
GATA-2irGFP 3^†	0.38	3	1.15

Mouse	Total Sca⁺ Lin⁻ Cells^‡ (×10⁵)	% GFP ⁺	Total GFP⁺ Sca⁺ Lin⁻ Cells (×10³)
irGFP 1^*	1.08	75	81
irGFP 2^*	1.20	84	101
irGFP 3^†	2.20	85	191
GATA-2irGFP 1^*	0.17	14	2.38
GATA-2irGFP 6^*	0.16	24	3.84
GATA-2irGFP 3^†	0.38	3	1.15

Lethally irradiated mice were transplanted with purified populations of GATA-2irGFP vector- (3 × 10⁶ cells per mouse) and control irGFP vector-expressing marrow cells (2 × 10⁶ cells per mouse). Mice were killed and bone marrow from hind limb bones analyzed at the times indicated.

Analysis performed 5-weeks post-transplantation.

†

Analysis performed 7-weeks post-transplantation.

‡

Number of cells contained in the two tibia and femur hind limb bones.

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