Effect of Combined Treatment of As2O3 and Ascorbic Acid of Primary Cultures of Chronic Lymphocytic Leukemia Cells
| Patient No. . | Viability (%) . | ||
|---|---|---|---|
| 1 Day . | 3 Days . | 5 Days . | |
| 1 | |||
| Control | 93 | 93 | 78 |
| As2O3 (1 μmol/L) | 92 | 86 | 68 |
| AA (125 μmol/L) | 85 | 78 | 74 |
| As2O3 (1 μmol/L) + AA (125 μmol/L) | 74 | 53 | 32 |
| 2 | |||
| Control | 88 | 86 | 80 |
| As2O3 (1 μmol/L) | 85 | 84 | 76 |
| AA (125 μmol/L) | 87 | 73 | 72 |
| As2O3 (1 μmol/L) + AA (125 μmol/L) | 62 | 41 | 36 |
| 3 | |||
| Control | 91 | 89 | 81 |
| As2O3 (1 μmol/L) | 93 | 81 | 69 |
| AA (125 μmol/L) | 71 | 70 | 70 |
| As2O3 (1 μmol/L) + AA (125 μmol/L) | 63 | 46 | 41 |
| Patient No. . | Viability (%) . | ||
|---|---|---|---|
| 1 Day . | 3 Days . | 5 Days . | |
| 1 | |||
| Control | 93 | 93 | 78 |
| As2O3 (1 μmol/L) | 92 | 86 | 68 |
| AA (125 μmol/L) | 85 | 78 | 74 |
| As2O3 (1 μmol/L) + AA (125 μmol/L) | 74 | 53 | 32 |
| 2 | |||
| Control | 88 | 86 | 80 |
| As2O3 (1 μmol/L) | 85 | 84 | 76 |
| AA (125 μmol/L) | 87 | 73 | 72 |
| As2O3 (1 μmol/L) + AA (125 μmol/L) | 62 | 41 | 36 |
| 3 | |||
| Control | 91 | 89 | 81 |
| As2O3 (1 μmol/L) | 93 | 81 | 69 |
| AA (125 μmol/L) | 71 | 70 | 70 |
| As2O3 (1 μmol/L) + AA (125 μmol/L) | 63 | 46 | 41 |
Lymphocytes (>95 of the cells) were isolated as described in the Methods from peripheral blood obtained from patients with CLL at various stages of disease. Values are the mean of 3 independent experiments.