Table 1. Neutrophil-Associated Proteins... | American Society of Hematology

Table 1.

Neutrophil-Associated Proteins in Neutrophilic Cells Grown by Various Combinations of Growth Factors From CD34⁺ Cord Blood Cells in Serum-Deprived Liquid Culture

Growth Factors	MPO	Elastase	Lactoferrin	CD11b	CD35	ALP
Exp. 1 (at 3 weeks)
SCF + TPO	+	+	+	+	−	−
SCF + G-CSF	+	+	+	+	+	+
SCF + GM-CSF	+	+	+	+	+	−
Exp. 2 (at 8 weeks)
SCF + TPO	+	+	+	+	−	−
SCF + TPO + G-CSF	+	+	+	+	+	+
SCF + TPO + GM-CSF	+	+	+	+	+	−

Growth Factors	MPO	Elastase	Lactoferrin	CD11b	CD35	ALP
Exp. 1 (at 3 weeks)
SCF + TPO	+	+	+	+	−	−
SCF + G-CSF	+	+	+	+	+	+
SCF + GM-CSF	+	+	+	+	+	−
Exp. 2 (at 8 weeks)
SCF + TPO	+	+	+	+	−	−
SCF + TPO + G-CSF	+	+	+	+	+	+
SCF + TPO + GM-CSF	+	+	+	+	+	−

For the examination of neutrophil-associated proteins in neutrophilic cells grown by SCF + TPO from CD34⁺ cord blood cells, the cells generated at 3 weeks or at 8 weeks in culture were harvested and recultured with various combinations of growth factors. After 1 week, the cells were processed for staining with MoAbs for MPO, elastase, lactoferrin, CD11b, and CD35 or with ALP. The results shown are from one representative experiment of three. Similar results were obtained in the other two experiments.

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