Checkerboard Analysis of Purified 5T2 MM Cells
| IGF-1 (bottom compartment) . | Upper Compartment . | ||
|---|---|---|---|
| 0 ng/mL . | 5 ng/mL . | 10 ng/mL . | |
| 0 ng/mL | BV | 60 | 10 |
| 5 ng/mL | 33 | 70 | 50 |
| 10 ng/mL | 50 | 82 | 64 |
| Bone Marrow– Conditioned Medium (bottom compartment) | Upper Compartment | ||
| 0× | 5× | 10× | |
| 0× | BV | 52 | 42 |
| 5× | 47 | 76 | 52 |
| 10× | 61 | 95 | 66 |
| Bone Marrow– Conditioned Medium + 10 μg/mL Anti–IGF-1 (bottom compartment) | Upper Compartment | ||
| 0× | 5× | 10× | |
| 0× | BV | 1 | 1 |
| 5× | 1 | 1 | 1 |
| 10× | 1 | 1 | 1 |
| IL-6 (bottom compartment) | Upper Compartment | ||
| 0 ng/mL | 5 ng/mL | 10 ng/mL | |
| 0 ng/mL | BV | 1 | 1 |
| 5 ng/mL | 1 | 1 | 1 |
| 10 ng/mL | 1 | 1 | 2 |
| Skin Fibroblast– Conditioned Medium (bottom compartment) | Upper Compartment | ||
| 0× | 5× | 10× | |
| 0× | BV | 1 | 22 |
| 5× | 1 | 3 | 26 |
| 10× | 1 | 1 | 22 |
| IGF-1 (bottom compartment) . | Upper Compartment . | ||
|---|---|---|---|
| 0 ng/mL . | 5 ng/mL . | 10 ng/mL . | |
| 0 ng/mL | BV | 60 | 10 |
| 5 ng/mL | 33 | 70 | 50 |
| 10 ng/mL | 50 | 82 | 64 |
| Bone Marrow– Conditioned Medium (bottom compartment) | Upper Compartment | ||
| 0× | 5× | 10× | |
| 0× | BV | 52 | 42 |
| 5× | 47 | 76 | 52 |
| 10× | 61 | 95 | 66 |
| Bone Marrow– Conditioned Medium + 10 μg/mL Anti–IGF-1 (bottom compartment) | Upper Compartment | ||
| 0× | 5× | 10× | |
| 0× | BV | 1 | 1 |
| 5× | 1 | 1 | 1 |
| 10× | 1 | 1 | 1 |
| IL-6 (bottom compartment) | Upper Compartment | ||
| 0 ng/mL | 5 ng/mL | 10 ng/mL | |
| 0 ng/mL | BV | 1 | 1 |
| 5 ng/mL | 1 | 1 | 1 |
| 10 ng/mL | 1 | 1 | 2 |
| Skin Fibroblast– Conditioned Medium (bottom compartment) | Upper Compartment | ||
| 0× | 5× | 10× | |
| 0× | BV | 1 | 22 |
| 5× | 1 | 3 | 26 |
| 10× | 1 | 1 | 22 |
Each analysis is representative for 3 independent experiments. For bone marrow– and skin fibroblast–conditioned medium, dilutions of the 10× concentrated medium are shown. For IGF-1 and IL-6, 5 and 10 ng/mL are used. In the assay combining bone marrow–conditioned medium and anti–IGF-1 blocking antibodies, dilutions of 10× concentrated medium are combined with a constant concentration of 10 μg/mL anti–IGF-1. BV (basic value) represents the spontaneous migration of the MM cells without any added stimulus. The other values are the % increase of this basic value. Vertical arrows indicate the chemotactic effect.