The Reversibility of Heparin’s Effect on Dynamic Measurements of Bone Mineralization
| . | Labeled Surface (%) . | Mineral Apposition Rate (μm/day) . | Bone Formation Rate BoneSurface Based (μm3/μm2/d) . |
|---|---|---|---|
| Baseline | — | — | — |
| Day 28 | |||
| Control | 18.1 ± 2.0 | 1.44 ± 0.01 | 25.9 ± 2.7 |
| Heparin (0.5 U/g) | 14.9 ± 1.4 | 1.52 ± 0.02 | 24.5 ± 2.7 |
| Heparin (1.0 U/g) | 12.8 ± 1.1* | 1.52 ± 0.04 | 19.2 ± 2.2* |
| Day 56 | |||
| Control | 18.1 ± 2.0 | 1.44 ± 0.01 | 25.9 ± 2.7 |
| Heparin (0.5 U/g) | 16.9 ± 1.0 | 1.41 ± 0.06 | 24.3 ± 2.5 |
| Heparin (1.0 U/g) | 12.7 ± 1.4*,† | 1.49 ± 0.05 | 18.4 ± 2.3*,† |
| . | Labeled Surface (%) . | Mineral Apposition Rate (μm/day) . | Bone Formation Rate BoneSurface Based (μm3/μm2/d) . |
|---|---|---|---|
| Baseline | — | — | — |
| Day 28 | |||
| Control | 18.1 ± 2.0 | 1.44 ± 0.01 | 25.9 ± 2.7 |
| Heparin (0.5 U/g) | 14.9 ± 1.4 | 1.52 ± 0.02 | 24.5 ± 2.7 |
| Heparin (1.0 U/g) | 12.8 ± 1.1* | 1.52 ± 0.04 | 19.2 ± 2.2* |
| Day 56 | |||
| Control | 18.1 ± 2.0 | 1.44 ± 0.01 | 25.9 ± 2.7 |
| Heparin (0.5 U/g) | 16.9 ± 1.0 | 1.41 ± 0.06 | 24.3 ± 2.5 |
| Heparin (1.0 U/g) | 12.7 ± 1.4*,† | 1.49 ± 0.05 | 18.4 ± 2.3*,† |
Rats were injected daily with vehicle alone or unfractionated heparin as described in Materials and Methods. On days 28 and 56, rats were exsanguinated and their right femurs removed for histologic examination. To permit measurements of bone apposition rates and the calculation of dynamic parameters, all animals received two interperitoneal injections of fluorescent markers. The first, demeclocycline (15 mg/kg), was given 10 days before sacrifice, while the second, calcein (8 mg/kg), was given 3 days before sacrifice. Data are expressed as mean ± SEM.
P < .01 when compared with control values.
P > .05 when compared with values obtained from heparin-treated animals at day 28.