Mean Fluorescence Intensity (MFI) Values of CD80, CD86, and CD72 Histograms on AM and CD28 and CD152 Histograms on BAL T Cells in a Time Course Evaluation Test
| Alveolar Macrophages . | ||||||
|---|---|---|---|---|---|---|
| Time 0 | Medium Cultured | Stimulated | ||||
| TNF-α | IL-15 | IL-2 | IFN-γ | |||
| CD80 | 86.7 ± 14.1 | 10.7 ± 4.1 | 16.2 ± 5.1 | 84.2 ± 17.6 | 10.1 ± 2.2 | 80.1 ± 13.3 |
| CD86 | 142.6 ± 36.1 | 16.1 ± 5.0 | 18.8 ± 7.3 | 126.4 ± 22.2 | 14.6 ± 4.1 | 111.8 ± 33.6 |
| CD72 | 69.5 ± 6.5 | 19.3 ± 4.1 | 22.1 ± 4.4 | 20.9 ± 6.4 | 33.7 ± 4.9 | 29.0 ± 8.0 |
| Lung Lymphocytes | ||||||
| Time 0 | Medium Cultured | Stimulated | ||||
| TNF-α | IL-15 | IL-2 | IFN-γ | |||
| CD28 | 46.4 ± 14.1 | 17.1 ± 6.6 | 16.8 ± 7.0 | 36.6 ± 7.3 | 30.1 ± 12.2 | 38.9 ± 6.9 |
| CD152 | 6.1 ± 0.4 | 9.0 ± 1.1 | 3.9 ± 0.7 | 3.2 ± 0.7 | 6.9 ± 1.6 | 9.1 ± 4.1 |
| Alveolar Macrophages . | ||||||
|---|---|---|---|---|---|---|
| Time 0 | Medium Cultured | Stimulated | ||||
| TNF-α | IL-15 | IL-2 | IFN-γ | |||
| CD80 | 86.7 ± 14.1 | 10.7 ± 4.1 | 16.2 ± 5.1 | 84.2 ± 17.6 | 10.1 ± 2.2 | 80.1 ± 13.3 |
| CD86 | 142.6 ± 36.1 | 16.1 ± 5.0 | 18.8 ± 7.3 | 126.4 ± 22.2 | 14.6 ± 4.1 | 111.8 ± 33.6 |
| CD72 | 69.5 ± 6.5 | 19.3 ± 4.1 | 22.1 ± 4.4 | 20.9 ± 6.4 | 33.7 ± 4.9 | 29.0 ± 8.0 |
| Lung Lymphocytes | ||||||
| Time 0 | Medium Cultured | Stimulated | ||||
| TNF-α | IL-15 | IL-2 | IFN-γ | |||
| CD28 | 46.4 ± 14.1 | 17.1 ± 6.6 | 16.8 ± 7.0 | 36.6 ± 7.3 | 30.1 ± 12.2 | 38.9 ± 6.9 |
| CD152 | 6.1 ± 0.4 | 9.0 ± 1.1 | 3.9 ± 0.7 | 3.2 ± 0.7 | 6.9 ± 1.6 | 9.1 ± 4.1 |
Data are reported as mean ± SE of the MFI values after subtracting the MFI of isotype control MoAb from the MFI of positively stained samples. Bold numbers denote the significant values with respect to the medium cultured AMs and T lymphocytes.