Solubilized HCA Interaction With Hematopoietic and Stromal Cells
| Step 1: Formation of HCA/Antibody Complexes3-150 . | Step 2: Selection of Intact Cells3-151 . | ||
|---|---|---|---|
| Antibody . | Cell Source . | Target Cells . | % Cells Bound to Complexes . |
| F84.1 | L2Ori− 3-152 | L2Ori− | 20; 25 (2 exp.) |
| Irrelevant | L2Ori− | L2Ori− | 0, 0 (2 exp.) |
| F84.1 | L2Ori− | PBL | 30 (1 exp.) |
| Irrelevant | L2Ori− | PBL | 0 (1 exp.) |
| F84.1 | Stro-1+ cell-derived stroma | Stro-1+ cell-derived stroma | 20; 22 (2 exp.) |
| Irrelevant | Stro-1+ cell-derived stroma | Stro-1+ cell-derived stroma | 0; 0 (2 exp.) |
| F84.1 | Stro-1+ cell-derived stroma | CD34+ | 11; 10; 11 (3 exp.) |
| Irrelevant | Stro-1+ cell-derived stroma | CD34+ | 0; 0; 0 (3 exp.) |
| F84.1 | Stro-1+cell-derived stroma | K5623-153 | 0; 0 (2 exp.) |
| Step 1: Formation of HCA/Antibody Complexes3-150 . | Step 2: Selection of Intact Cells3-151 . | ||
|---|---|---|---|
| Antibody . | Cell Source . | Target Cells . | % Cells Bound to Complexes . |
| F84.1 | L2Ori− 3-152 | L2Ori− | 20; 25 (2 exp.) |
| Irrelevant | L2Ori− | L2Ori− | 0, 0 (2 exp.) |
| F84.1 | L2Ori− | PBL | 30 (1 exp.) |
| Irrelevant | L2Ori− | PBL | 0 (1 exp.) |
| F84.1 | Stro-1+ cell-derived stroma | Stro-1+ cell-derived stroma | 20; 22 (2 exp.) |
| Irrelevant | Stro-1+ cell-derived stroma | Stro-1+ cell-derived stroma | 0; 0 (2 exp.) |
| F84.1 | Stro-1+ cell-derived stroma | CD34+ | 11; 10; 11 (3 exp.) |
| Irrelevant | Stro-1+ cell-derived stroma | CD34+ | 0; 0; 0 (3 exp.) |
| F84.1 | Stro-1+cell-derived stroma | K5623-153 | 0; 0 (2 exp.) |
Abbreviations: PBL, peripheral blood lymphocytes; exp., experiments.
Complexes formed between F84.1 antibody-coated beads and membrane molecules, as described in Materials and Methods.
Intact cells were incubated with the complexes as described in Materials and Methods.
The L2Ori− stromal cell line was used in this experiment because of its high HCA antigen expression (see Table 1).
Negative control.