Cytokine Production by HO-1 in Response to Peptide Stimulation
| Cells Cultured . | Peptide Stimulation . | Cytokine Production (pg/mL) . | |||
|---|---|---|---|---|---|
| IL-4 . | IL-10 . | TNF-α . | IFN-γ . | ||
| HO-1 + Auto APC | + | 762 | 1,558 | 1,524 | 3,088 |
| − | 0 | 43 | 73 | 166 | |
| Auto APC | + | 0 | 26 | 64 | 0 |
| Cells Cultured . | Peptide Stimulation . | Cytokine Production (pg/mL) . | |||
|---|---|---|---|---|---|
| IL-4 . | IL-10 . | TNF-α . | IFN-γ . | ||
| HO-1 + Auto APC | + | 762 | 1,558 | 1,524 | 3,088 |
| − | 0 | 43 | 73 | 166 | |
| Auto APC | + | 0 | 26 | 64 | 0 |
HO-1 cells were cultured in the presence of autologous APCs with or without DEK-CAN peptide for 72 hours, and the concentration of each cytokine in the culture supernatant was determined by ELISA.