Binding of Affinity Purified Phage Clones to PMN, Peripheral Blood Lymphocytes, and Cell Lines
| . | Phage Peptide Sequences . | Control Phage . | ||
|---|---|---|---|---|
| DLVTSKLQV . | FGPNLTGRW . | CKDGLFLGSWLC . | ||
| PMN* | +† | + | +‡ | − |
| Monocytic cells1-153 | − | + | + | − |
| Lymphocytes1-155 | − | − | − | − |
| Epithelial cells1-154 | − | − | − | − |
| Endothelial cells# | − | − | − | − |
| Fibroblasts1-160 | − | − | − | − |
| . | Phage Peptide Sequences . | Control Phage . | ||
|---|---|---|---|---|
| DLVTSKLQV . | FGPNLTGRW . | CKDGLFLGSWLC . | ||
| PMN* | +† | + | +‡ | − |
| Monocytic cells1-153 | − | + | + | − |
| Lymphocytes1-155 | − | − | − | − |
| Epithelial cells1-154 | − | − | − | − |
| Endothelial cells# | − | − | − | − |
| Fibroblasts1-160 | − | − | − | − |
Experiments were performed at least twice. As a positive control, cells were labeled with anti-CD47.
Isolated from human venous blood.
Approximately 50% of the PMN labeled.
Cells labeled at 20°C only; no labeling was observed at 4°C.
Human U937 monocytic cell line.
Isolated from human peripheral venous blood.
Human intestinal cell lines T84 and HT29.
#Transformed endothelial cells ECV 304.
VA2 fibroblasts.