Effect of PA317 Supernatants Derived From Transfection With Various Vpr Mutants
| Vpr Mutation . | Supernatant RT Activity (dpm) . | Phagocyte Activation . |
|---|---|---|
| WT | 4,200 | +++ |
| A59P | 5,600 | −/+ |
| R62S | 4,700 | ++ |
| L64S | 3,300 | ++ |
| H71C | 4,400 | +++ |
| C76A | 4,200 | ++ |
| HXB2 | 4,300 | −/+ |
| Control media | 200 | − |
| Vpr Mutation . | Supernatant RT Activity (dpm) . | Phagocyte Activation . |
|---|---|---|
| WT | 4,200 | +++ |
| A59P | 5,600 | −/+ |
| R62S | 4,700 | ++ |
| L64S | 3,300 | ++ |
| H71C | 4,400 | +++ |
| C76A | 4,200 | ++ |
| HXB2 | 4,300 | −/+ |
| Control media | 200 | − |
Murine marrow cultures were treated with supernatants derived from transfections with mutants of Vpr (in HIV-1 strain 86.9) and were also assayed for reverse transcriptase activity. After addition of supernatants, cultures were assessed for their state of activation (erythrophagocytosis).
Abbreviations: +++, wt Vpr activity; −/+, low number of phagocytic islets; −, no phagocytic islets observed.