Effect of VN on Thrombin Time
| Pooled Plasma . | Thrombin Time (seconds) . | P Value . |
|---|---|---|
| VN +/+ (n = 4) | 34.2 ± 6.7 | |
| VN −/− (n = 4) | 20.5 ± 2.1 | <.01 v VN +/+ |
| VN−/− plus purified VN (n = 3) | 41.2 ± 5.5 | <.001 v VN −/− |
| Pooled Plasma . | Thrombin Time (seconds) . | P Value . |
|---|---|---|
| VN +/+ (n = 4) | 34.2 ± 6.7 | |
| VN −/− (n = 4) | 20.5 ± 2.1 | <.01 v VN +/+ |
| VN−/− plus purified VN (n = 3) | 41.2 ± 5.5 | <.001 v VN −/− |
Thrombin times were performed by mixing 100 μL of citrated pooled plasma, 55.5 μL of BSA or VN (1.3 mg/mL in PBS), and 50 μL of thrombin (18 U/mL) and measuring time to clot formation at 37°C.