vCWRHIVAPAP Sequences in Individual Colonies From LTC-IC Assays
| Donor . | Week-5 LTC-IC . | Week-8 LTC-IC . | Week-10 LTC-IC . |
|---|---|---|---|
| FF* | 42% (5/12) | — | — |
| BE* | 7% (1/15) | — | — |
| CL | 100% (10/10) | — | — |
| TS | 80% (16/20) | 60% (9/15) | — |
| FK | 69% (9/13) | — | 67% (8/12) |
| HJ* | 36% (4/11) | 67% (6/9) | 60% (6/10) |
| SM* | 5% (1/20) | 20% (4/20) | 25% (4/16) |
| Donor . | Week-5 LTC-IC . | Week-8 LTC-IC . | Week-10 LTC-IC . |
|---|---|---|---|
| FF* | 42% (5/12) | — | — |
| BE* | 7% (1/15) | — | — |
| CL | 100% (10/10) | — | — |
| TS | 80% (16/20) | 60% (9/15) | — |
| FK | 69% (9/13) | — | 67% (8/12) |
| HJ* | 36% (4/11) | 67% (6/9) | 60% (6/10) |
| SM* | 5% (1/20) | 20% (4/20) | 25% (4/16) |
Cells were transduced on day 0 at functional MOI of 3 (particle MOI, 600) as described in Materials and Methods. Cells harvested from stromal cultures initiated with marrow-derived CD34 cells were assayed for LTC-ICs at 5, 8, and 10 weeks. Individual colonies were plucked, DNA-isolated, and amplified with primers specific for vector and β globin. The total number of vector positive colonies of all beta globin positive colonies is shown.
Abbreviation: —, no LTC-ICs were detected at those time points from those donors.
Donors with concurrent FISH analyses.