Table 1.

Cellular Components of the Newborn Mouse Spleens

 No. of Nucleated Cells/Spleen% of Nucleated CellsNo. of Erythrocytes/ Spleen
CD3+CD4+CD8+Mac-1+B220+CD3− Mac-1− B220−
Nor-1 1.94 × 106 1.0 0.3 0.1 19.1 14.7 65.2 2.56 × 106 
Nor-2 1.78 × 106 1.2 0.3 0.3 17.7 24.9 56.2 2.72 × 106 
KO-1 0.46 × 106 2.6 1.2 0.6 13.9 13.8 69.7 0.58 × 106 
KO-2 0.34 × 106 0.9 0.3 0.4 14.2 14.5 70.4 0.54 × 106 
 No. of Nucleated Cells/Spleen% of Nucleated CellsNo. of Erythrocytes/ Spleen
CD3+CD4+CD8+Mac-1+B220+CD3− Mac-1− B220−
Nor-1 1.94 × 106 1.0 0.3 0.1 19.1 14.7 65.2 2.56 × 106 
Nor-2 1.78 × 106 1.2 0.3 0.3 17.7 24.9 56.2 2.72 × 106 
KO-1 0.46 × 106 2.6 1.2 0.6 13.9 13.8 69.7 0.58 × 106 
KO-2 0.34 × 106 0.9 0.3 0.4 14.2 14.5 70.4 0.54 × 106 

Cells prepared from the spleens of the wild-type (Nor-1 and Nor-2) and the mFtz-F1 gene–disrupted mice (KO-1 and KO-2) were used for detection of cell surface markers, CD3, CD4, CD8, Mac-1, and B220, as described in Materials and Methods. Numbers of the nucleated cells and erythrocytes were counted under the microscope.

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