Effect of rmSCF on Proportions of CMCs Expressing MMCP-4, MMCP-5, or MMCP-6 mRNA in +/+ and mi/mi CMCs Shown by In Situ Hybridization
| Genotype of CMCs . | Concentration of rmSCF (ng/mL)* . | Proportion of MMCP-4 mRNA+ to Alcian Blue+ Cells (%)† . | Proportion of MMCP-5 mRNA+ to Alcian Blue+ Cells (%)† . | Proportion of MMCP-6 mRNA+ to Alcian Blue+ Cells (%)† . |
|---|---|---|---|---|
| +/+ | 0 | 23 ± 4 | 47 ± 2 | 46 ± 1 |
| mi/mi | 0 | 5 ± 1‡ | 4 ± 3‡ | 6 ± 1‡ |
| +/+ | 10 | 29 ± 2 | 55 ± 6 | 51 ± 3 |
| mi/mi | 10 | 6 ± 1‡ | 45 ± 42-153 | 5 ± 2‡ |
| +/+ | 50 | 30 ± 3 | 50 ± 5 | 52 ± 2 |
| mi/mi | 50 | 7 ± 2‡ | 40 ± 42-153 | 6 ± 1‡ |
| Genotype of CMCs . | Concentration of rmSCF (ng/mL)* . | Proportion of MMCP-4 mRNA+ to Alcian Blue+ Cells (%)† . | Proportion of MMCP-5 mRNA+ to Alcian Blue+ Cells (%)† . | Proportion of MMCP-6 mRNA+ to Alcian Blue+ Cells (%)† . |
|---|---|---|---|---|
| +/+ | 0 | 23 ± 4 | 47 ± 2 | 46 ± 1 |
| mi/mi | 0 | 5 ± 1‡ | 4 ± 3‡ | 6 ± 1‡ |
| +/+ | 10 | 29 ± 2 | 55 ± 6 | 51 ± 3 |
| mi/mi | 10 | 6 ± 1‡ | 45 ± 42-153 | 5 ± 2‡ |
| +/+ | 50 | 30 ± 3 | 50 ± 5 | 52 ± 2 |
| mi/mi | 50 | 7 ± 2‡ | 40 ± 42-153 | 6 ± 1‡ |
+/+ or mi/mi CMCs were incubated with 10% PWM-SCM and various concentrations of rmSCF for 2 weeks.
Mean ± SE of five independent experiments.
P < .01 by t-test when compared with the value of +/+ CMCs, which were cultured without rmSCF.
P < .01 by t-test when compared with the value ofmi/mi CMCs, which were cultured without rmSCF.