Human Prothrombin and β2-Glycoprotein I Binding to Phospholipids Depends on Phospholipid Charge and Conformation
| Phospholipids . | Absorbance (mOD, 405 nm) . | ||
|---|---|---|---|
| hPT/Ca2+ . | hPT . | β2-GPI . | |
| PC | 6 | 98 | 1 |
| PS | 1,971 | 6 | 1,994 |
| Bovine brain PE | 103 | 94 | 17 |
| Staclot LA | 810 | 94 | 0 |
| Phospholipids . | Absorbance (mOD, 405 nm) . | ||
|---|---|---|---|
| hPT/Ca2+ . | hPT . | β2-GPI . | |
| PC | 6 | 98 | 1 |
| PS | 1,971 | 6 | 1,994 |
| Bovine brain PE | 103 | 94 | 17 |
| Staclot LA | 810 | 94 | 0 |
Results are expressed as mean value of two replicates. Bovine brain PE was used as a source of lamellar PE; Staclot LA was used as a source of hexagonal II PE.
Abbreviations: PC, phasphatidylcholine; PS, phosphatidylserine; PE, phosphatidylethanolamine. β2-GPI, β2-glycoprotein I; Ca2+, calcium ions; hPT, human prothrombin.