Surface Phenotype Induced in Sorted Cells After 3, 7, and 11 Days of Incubation With Different Cytokines
| . | Total CD1a+ . | CD1a+CD14− . | CD1a−CD14+ . | CD83 . | CD80 . | CD1a+/Dex+ . |
|---|---|---|---|---|---|---|
| p14+ cells | ||||||
| DC induction | ||||||
| 3 d G4 | 78 ± 5.6 | 74 ± 3.5 | 1.8 ± 1.5 | 6.2 ± 3.6 | 5.2 ± 2.5 | 62 ± 1.0 |
| 3 d G4, 4 d GT4 | 86 ± 3.5 | 84 ± 3.9 | 0.6 ± 0.2 | 67 ± 1.5 | 49 ± 17 | 31 ± 3.0 |
| p14− cells | ||||||
| Direct DC induction | ||||||
| 3 d GT4 | 57 ± 15 | 49 ± 11 | 2.6 ± 2.4 | 12 ± 1.6 | 8.7 ± 2.2 | 42 ± 8.0 |
| 7 d GT4 | 61 ± 16 | 60 ± 15 | 1.0 ± 0.9 | 23 ± 12 | 30 ± 3.0 | 25 ± 12 |
| Monocytic induction | ||||||
| 7 d GT | 9.8 ± 6.2 | 3.9 ± 0.9 | 78 ± 6.7 | 2.9 ± 2.2 | 9.5 ± 2.4 | ND |
| 7 d GF | 7.1 ± 3.8 | 1.0 ± 0.8 | 71 ± 7.0 | 2.8 ± 1.4 | 7.7 ± 1.3 | ND |
| Indirect DC induction | ||||||
| 3 d GT, 4 d G4 | 45 ± 22 | 35 ± 15 | 21 ± 9.4 | 6.7 ± 3.3 | 18 ± 11 | 69 ± 6.0 |
| 3 d GF, 4 d G4 | 41 ± 10 | 31 ± 9.8 | 12 ± 9.6 | 5.4 ± 1.2 | 20 ± 8.2 | 81 ± 11 |
| 3 d GF, 4 d GF4 | 39 ± 13 | 31 ± 13 | 14 ± 11 | 8.0 ± 4.5 | 18 ± 8.1 | 73 ± 8.0 |
| 3 d GF, 4 d G4, 4 d GT4 | 72 ± 15 | 71 ± 16 | 1.2 ± 1.5 | 26 ± 6.8 | 28 ± 1.6 | 28 ± 8.7 |
| 3 d GF, 4 d GF4, 4 d GFT4 | 71 ± 12 | 71 ± 13 | 0.7 ± 0.4 | 24 ± 8.4 | 31 ± 22 | 28 ± 9.9 |
| . | Total CD1a+ . | CD1a+CD14− . | CD1a−CD14+ . | CD83 . | CD80 . | CD1a+/Dex+ . |
|---|---|---|---|---|---|---|
| p14+ cells | ||||||
| DC induction | ||||||
| 3 d G4 | 78 ± 5.6 | 74 ± 3.5 | 1.8 ± 1.5 | 6.2 ± 3.6 | 5.2 ± 2.5 | 62 ± 1.0 |
| 3 d G4, 4 d GT4 | 86 ± 3.5 | 84 ± 3.9 | 0.6 ± 0.2 | 67 ± 1.5 | 49 ± 17 | 31 ± 3.0 |
| p14− cells | ||||||
| Direct DC induction | ||||||
| 3 d GT4 | 57 ± 15 | 49 ± 11 | 2.6 ± 2.4 | 12 ± 1.6 | 8.7 ± 2.2 | 42 ± 8.0 |
| 7 d GT4 | 61 ± 16 | 60 ± 15 | 1.0 ± 0.9 | 23 ± 12 | 30 ± 3.0 | 25 ± 12 |
| Monocytic induction | ||||||
| 7 d GT | 9.8 ± 6.2 | 3.9 ± 0.9 | 78 ± 6.7 | 2.9 ± 2.2 | 9.5 ± 2.4 | ND |
| 7 d GF | 7.1 ± 3.8 | 1.0 ± 0.8 | 71 ± 7.0 | 2.8 ± 1.4 | 7.7 ± 1.3 | ND |
| Indirect DC induction | ||||||
| 3 d GT, 4 d G4 | 45 ± 22 | 35 ± 15 | 21 ± 9.4 | 6.7 ± 3.3 | 18 ± 11 | 69 ± 6.0 |
| 3 d GF, 4 d G4 | 41 ± 10 | 31 ± 9.8 | 12 ± 9.6 | 5.4 ± 1.2 | 20 ± 8.2 | 81 ± 11 |
| 3 d GF, 4 d GF4 | 39 ± 13 | 31 ± 13 | 14 ± 11 | 8.0 ± 4.5 | 18 ± 8.1 | 73 ± 8.0 |
| 3 d GF, 4 d G4, 4 d GT4 | 72 ± 15 | 71 ± 16 | 1.2 ± 1.5 | 26 ± 6.8 | 28 ± 1.6 | 28 ± 8.7 |
| 3 d GF, 4 d GF4, 4 d GFT4 | 71 ± 12 | 71 ± 13 | 0.7 ± 0.4 | 24 ± 8.4 | 31 ± 22 | 28 ± 9.9 |
G4: GM-CSF plus IL-4; GT4: GM-CSF plus IL-4 and TNF; GT: GM-CSF plus TNF; GF: GM-CSF plus FLT3-L; GF4: GM-CSF plus FLT3-L and IL-4; GFT4: as previously plus TNF. These data are the mean ± SD of at least three experiments for CD determination and are the mean of two experiments for FITC-dextran internalization.
Abbreviation: ND, not determined.