Cell Cycle Analysis of UF-1 Cells
| Treatment . | % Fluorescence at Cell Cycle Phase: . | ||
|---|---|---|---|
| G0/G1 . | S . | G2/M . | |
| Control | 80.2 | 13.8 | 6.0 |
| All-transRA | 91.9 | 6.7 | 1.5 |
| 1,25(OH)2D3 | 93.3 | 5.0 | 1.7 |
| 1,25(OH)2D3+ all-trans RA | 98.4 | 0.5 | 1.1 |
| Treatment . | % Fluorescence at Cell Cycle Phase: . | ||
|---|---|---|---|
| G0/G1 . | S . | G2/M . | |
| Control | 80.2 | 13.8 | 6.0 |
| All-transRA | 91.9 | 6.7 | 1.5 |
| 1,25(OH)2D3 | 93.3 | 5.0 | 1.7 |
| 1,25(OH)2D3+ all-trans RA | 98.4 | 0.5 | 1.1 |
UF-1 cells were cultured with either 10−7 mol/L all-trans RA, 10−7 mol/L 1,25(OH)2D3, or a combination of both for 48 hours. DNA was stained with propidium iodide, and analysis was immediately performed using the CELLFIT program.