PCR Conditions of LCEA Clones
| Group No. . | Clone Name . | Primer Sequences . | Product Length (bp) . | Annealing Temperature (°C) . | MgCl2 Concentration (mmol/L) . |
|---|---|---|---|---|---|
| 1 | 12-1 | 5′ CAC TGA CTT GGG GAC TGC | 149 | 59 | 0.5 |
| 5′ GAG TTC CAC TTT CAG CCC (Yan et al,20 1995) | |||||
| 2 | 11-4 | 5′ CAT ACA GGG CAA AAT CAG AGC | 172 | 57 | 0.4 |
| 5′ TGA ATA ACA CTC ACT GCT GGC | |||||
| 3 | 51-2 | 5′ ATT TTG CCT CTT GCA CGG TC | 162 | 58 | 1.5 |
| 5′ CAG ATG CCC CTG GTC TTG (Heckel et al,22 1998) | |||||
| 4 | 39-1 | 5′ GCC CAC CTC CTT GTG TAT ATC | 157 | 56 | 1.5 |
| 5′ TTT CCT TCC AAG TTT AGC TGC | |||||
| 5 | 37-1 | 5′ ACA GGA ATT CTG GAG CCA CG | 152 | 60 | 1 |
| 5′ ATT TCC AGG AGC CGG AGA AG | |||||
| 6 | 26-4 | 5′ TCA GGG AAA TCA AGG ACT CTC | 140 | 56 | 2 |
| 5′ TTC AAG CTC CAG CAA CAT ATC | |||||
| 7 | 13-2 | 5′ ACA AAG TCA TCC CTT GCT GC | 138 | 58 | 1.5 |
| 5′ AGC ATT CAC CCA GTC CTC TG | |||||
| 24-2 | 5′ GAG AGC ATC CAG ACG TTC AG | 950 | 57 | 1 | |
| 5′ AAG TTG CTC CTC AAA GCC TC | |||||
| 25-3 | ND | ||||
| 31-1 | 5′ AGC TGA CAC AGA CAT CAA ACG | 174 | 58 | 1.5 | |
| 5′ CTC ACG GAA AGA AAC AAC CTG | |||||
| 44-1 | 5′ AAT GAG TCC AAT GCC TCT CC | 146 | 56 | 1 | |
| 5′ GAA GTT CCT GGT TCT CCA GC | |||||
| 9-1 | 5′ GCT TTA TTG CCC TTG TCT ATG C | 171 | 58 | 3.5 | |
| 5′ TCC TAT CTG CTT CTA CCA TCC C | |||||
| 11-2 | 5′ CAG TCC CAT CAA TAG GGG G | 225 | 60 | 2 | |
| 5′ GCA AGC CAT TCC TAA ACA GC | |||||
| 54-1 | ND | ||||
| 59-2 | 5′ GCT TTG GCT TTC ACT CTC TTG | 151 | 59 | 2 | |
| 5′ ACA GAA CCG TAT CAG CGA CC | |||||
| 61-2 | 5′ GGC CTG GAG GAG GAG ACT T | 134 | 59 | 4 | |
| 5′ CGT GGA GGA CGC TGA GTT | |||||
| 61-3 | ND | ||||
| 96-5 | 5′ TCC CAC TAA AAC AAG GCA CC | 143 | 59 | 2 | |
| 5′ GCA CCT CCT CAC ATT CGA CC | |||||
| 129-1 | 5′ AGA CTC GAC GGC AAT GAC TC | 150 | 58 | 1.5 | |
| 5′ GAA AGA CAG CCC AGC TCT TG |
| Group No. . | Clone Name . | Primer Sequences . | Product Length (bp) . | Annealing Temperature (°C) . | MgCl2 Concentration (mmol/L) . |
|---|---|---|---|---|---|
| 1 | 12-1 | 5′ CAC TGA CTT GGG GAC TGC | 149 | 59 | 0.5 |
| 5′ GAG TTC CAC TTT CAG CCC (Yan et al,20 1995) | |||||
| 2 | 11-4 | 5′ CAT ACA GGG CAA AAT CAG AGC | 172 | 57 | 0.4 |
| 5′ TGA ATA ACA CTC ACT GCT GGC | |||||
| 3 | 51-2 | 5′ ATT TTG CCT CTT GCA CGG TC | 162 | 58 | 1.5 |
| 5′ CAG ATG CCC CTG GTC TTG (Heckel et al,22 1998) | |||||
| 4 | 39-1 | 5′ GCC CAC CTC CTT GTG TAT ATC | 157 | 56 | 1.5 |
| 5′ TTT CCT TCC AAG TTT AGC TGC | |||||
| 5 | 37-1 | 5′ ACA GGA ATT CTG GAG CCA CG | 152 | 60 | 1 |
| 5′ ATT TCC AGG AGC CGG AGA AG | |||||
| 6 | 26-4 | 5′ TCA GGG AAA TCA AGG ACT CTC | 140 | 56 | 2 |
| 5′ TTC AAG CTC CAG CAA CAT ATC | |||||
| 7 | 13-2 | 5′ ACA AAG TCA TCC CTT GCT GC | 138 | 58 | 1.5 |
| 5′ AGC ATT CAC CCA GTC CTC TG | |||||
| 24-2 | 5′ GAG AGC ATC CAG ACG TTC AG | 950 | 57 | 1 | |
| 5′ AAG TTG CTC CTC AAA GCC TC | |||||
| 25-3 | ND | ||||
| 31-1 | 5′ AGC TGA CAC AGA CAT CAA ACG | 174 | 58 | 1.5 | |
| 5′ CTC ACG GAA AGA AAC AAC CTG | |||||
| 44-1 | 5′ AAT GAG TCC AAT GCC TCT CC | 146 | 56 | 1 | |
| 5′ GAA GTT CCT GGT TCT CCA GC | |||||
| 9-1 | 5′ GCT TTA TTG CCC TTG TCT ATG C | 171 | 58 | 3.5 | |
| 5′ TCC TAT CTG CTT CTA CCA TCC C | |||||
| 11-2 | 5′ CAG TCC CAT CAA TAG GGG G | 225 | 60 | 2 | |
| 5′ GCA AGC CAT TCC TAA ACA GC | |||||
| 54-1 | ND | ||||
| 59-2 | 5′ GCT TTG GCT TTC ACT CTC TTG | 151 | 59 | 2 | |
| 5′ ACA GAA CCG TAT CAG CGA CC | |||||
| 61-2 | 5′ GGC CTG GAG GAG GAG ACT T | 134 | 59 | 4 | |
| 5′ CGT GGA GGA CGC TGA GTT | |||||
| 61-3 | ND | ||||
| 96-5 | 5′ TCC CAC TAA AAC AAG GCA CC | 143 | 59 | 2 | |
| 5′ GCA CCT CCT CAC ATT CGA CC | |||||
| 129-1 | 5′ AGA CTC GAC GGC AAT GAC TC | 150 | 58 | 1.5 | |
| 5′ GAA AGA CAG CCC AGC TCT TG |
The conditions were used for chromosomal localization and amplification analysis by PCR.
Abbreviation: ND, not determined, for these cDNA clones no functional primer pairs are available.