Oligonucleotides Used in the Construction of the β3 Mutants
| IIIa3 | GAG CTG ATC CCA GGG ACC AC |
| IIIa4-AfIII | GCA TCC TTG CCA GTG TCC TTA AG |
| T562A-sense | CTG CAA CTG TAC CGC GCG TAC TG |
| T562A-antisense | CAG TAC GCG CGG TAC AGT TGC AG |
| T562Q-sense | CTG CAA CTG TAC CCA GCG TAC TG |
| T562Q-antisense | CAG TAC GCT GGG TAC AGT TGC AG |
| T564A-sense | CGT GCT GAC ACC TGC ATG TC |
| T564A-antisense | GAC ATG CAG GTG TCA GCA CG |
| IIIa3 | GAG CTG ATC CCA GGG ACC AC |
| IIIa4-AfIII | GCA TCC TTG CCA GTG TCC TTA AG |
| T562A-sense | CTG CAA CTG TAC CGC GCG TAC TG |
| T562A-antisense | CAG TAC GCG CGG TAC AGT TGC AG |
| T562Q-sense | CTG CAA CTG TAC CCA GCG TAC TG |
| T562Q-antisense | CAG TAC GCT GGG TAC AGT TGC AG |
| T564A-sense | CGT GCT GAC ACC TGC ATG TC |
| T564A-antisense | GAC ATG CAG GTG TCA GCA CG |
The bold letters indicate base changes from the wild-type sequence.