Effect of TGF-β Antibody on Numbers and Viability of mBMMC and on the Expression and Release of mMCP-1
| Treatment . | Cells (×105/mL) . | % Viability . | % Mast Cells . | % mMCP-1+ . | mMCP-1 (ng/mL) . |
|---|---|---|---|---|---|
| W/S + αTGFβ Ab | 4.8 ± 0.5 | 92 ± 1 | 90 ± 1 | 0.5 ± 0.1 | 0 ± 0 |
| W/S + N. Chicken Ab | 5.4 ± 0.2 | 91 ± 1 | 88 ± 2 | 0.9 ± 0.4 | 0 ± 0 |
| W/S + no Ab | 6.0 ± 0.4 | 94 ± 1 | 92 ± 1 | 1.3 ± 0.3 | 0 ± 0 |
| I/S + αTGFβ Ab | 3.2 ± 0.3 | 87 ± 4 | 91 ± 0.5 | 1.1 ± 0.2a | 0 ± 0 |
| I/S + N. Chicken Ab | 4.5 ± 0.2 | 87 ± 1 | 95 ± 1 | 5.1 ± 0.3b | 0 ± 0 |
| I/S + no Ab | 4.3 ± 0.3 | 84 ± 2 | 91 ± 2 | 5.4 ± 1.3b | 0 ± 0 |
| T/I/W/S + αTGFβ Ab | 6.3 ± 0.1 | 90 ± 2 | 87 ± 1 | 0.6 ± 0.2c | 0 ± 0e |
| T/I/W/S + N. Chicken Ab | 7.0 ± 0.4 | 91 ± 2 | 88 ± 1 | 23 ± 0.5d | 15 ± 0.1f |
| T/I/W/S + no Ab | 5.7 ± 0.4 | 89 ± 3 | 89 ± 2 | 26 ± 1.9d | 14 ± 0.9f |
| Treatment . | Cells (×105/mL) . | % Viability . | % Mast Cells . | % mMCP-1+ . | mMCP-1 (ng/mL) . |
|---|---|---|---|---|---|
| W/S + αTGFβ Ab | 4.8 ± 0.5 | 92 ± 1 | 90 ± 1 | 0.5 ± 0.1 | 0 ± 0 |
| W/S + N. Chicken Ab | 5.4 ± 0.2 | 91 ± 1 | 88 ± 2 | 0.9 ± 0.4 | 0 ± 0 |
| W/S + no Ab | 6.0 ± 0.4 | 94 ± 1 | 92 ± 1 | 1.3 ± 0.3 | 0 ± 0 |
| I/S + αTGFβ Ab | 3.2 ± 0.3 | 87 ± 4 | 91 ± 0.5 | 1.1 ± 0.2a | 0 ± 0 |
| I/S + N. Chicken Ab | 4.5 ± 0.2 | 87 ± 1 | 95 ± 1 | 5.1 ± 0.3b | 0 ± 0 |
| I/S + no Ab | 4.3 ± 0.3 | 84 ± 2 | 91 ± 2 | 5.4 ± 1.3b | 0 ± 0 |
| T/I/W/S + αTGFβ Ab | 6.3 ± 0.1 | 90 ± 2 | 87 ± 1 | 0.6 ± 0.2c | 0 ± 0e |
| T/I/W/S + N. Chicken Ab | 7.0 ± 0.4 | 91 ± 2 | 88 ± 1 | 23 ± 0.5d | 15 ± 0.1f |
| T/I/W/S + no Ab | 5.7 ± 0.4 | 89 ± 3 | 89 ± 2 | 26 ± 1.9d | 14 ± 0.9f |
Data are expressed as the mean ± SE (n = 4). Statistical analysis shows that a versus b, c versus d, and e versus f are significantly different (P < .03 Mann Whitney). mBMMC were grown in WEHI (15%)/rrSCF (50 ng/mL) for 7 days before transferring them in quadruplicate to 48-well plates supplemented with rhTGF-β1 (100 pg/mL)/rmIL-9 (5 ng/mL)/WEHI (15%)/rrSCF (50 ng/mL) or with rmIL-9/rrSCF or WEHI/rrSCF at the same concentrations. These cytokines are denoted as follows: T, rhTGF-β1; I, rmIL-9; W, WEHI-3B IL-3–rich supernatant; S, rrSCF. Included in these cultures were chicken anti–TGF-β antibody (1 μg/mL), control chicken IgG antibody (1 μg/mL), or vehicle alone (no Ab) as indicated. The table shows the following parameters measured 2 days after transfer into cytokines: cell numbers and viability as assessed by nigrosin exclusion, percentage of mast cells in Leishman’s stained cytosmears, percentage of mMCP-1+ mast cells, and concentrations of mMCP-1 in culture supernatants (in nanograms per milliliter).