Effect of Inhibitors on Reduction of Cell Growth Induced by IFNγ
| Inhibitors–fmk . | Inhibitors–cho . | ||||
|---|---|---|---|---|---|
| Treatment . | % . | PValue . | Treatment . | % . | P Value . |
| Control | 100 ± 10 | Control | 100 ± 6 | ||
| IFNγ | 56 ± 4 | v Control <.01 | IFNγ | 66 ± 6 | v Control <.01 |
| IFNγ + IETD | 71 ± 3 | v IFNγ <.01 | IFNγ + YVAD | 87 ± 4 | v IFNγ <.01 |
| IFNγ + DEVD | 82 ± 3 | v IFNγ <.01 | IFNγ + DEVD | 88 ± 7 | v IFNγ <.01 |
| Inhibitors–fmk . | Inhibitors–cho . | ||||
|---|---|---|---|---|---|
| Treatment . | % . | PValue . | Treatment . | % . | P Value . |
| Control | 100 ± 10 | Control | 100 ± 6 | ||
| IFNγ | 56 ± 4 | v Control <.01 | IFNγ | 66 ± 6 | v Control <.01 |
| IFNγ + IETD | 71 ± 3 | v IFNγ <.01 | IFNγ + YVAD | 87 ± 4 | v IFNγ <.01 |
| IFNγ + DEVD | 82 ± 3 | v IFNγ <.01 | IFNγ + DEVD | 88 ± 7 | v IFNγ <.01 |
Two experiments with -fmk and three with -cho inhibitors were performed. Day-6 cells were preincubated for 2 hours with 40 μmol/L IETD-fmk or DEVD-fmk or 1 μmol/L YVAD-cho or DEVD-cho before adding 100 U/mL of IFNγ. After incubation for 96 hours, the cells were collected and counted. The number of cells in the group not treated with IFNγ was taken as 100% and the other groups were then compared with this control.