Efficiency of Transduction Into Marmoset Hematopoietic Progenitor Cells
| Transduction Method . | Cell . | Stroma . | Cytokine* . | Transduction Period . | ||
|---|---|---|---|---|---|---|
| 48 h . | 96 h . | 144 h . | ||||
| Supernatant | BMPC | − | + | ND | 1.7 ± 0.6 | ND |
| + | + | ND | 5.6 ± 1.2 | ND | ||
| Cocultivation | BMPC | − | − | 1.0 ± 0.6 | 3.2 ± 1.0 | 5.9 ± 0.9 |
| − | + | 1.6 ± 0.2 | 5.5 ± 0.5 | 6.3 ± 1.2 | ||
| + | − | 11.6 ± 1.5† | 15.3 ± 1.9† | 16.5 ± 1.2† | ||
| + | + | 15.2 ± 1.5† | 23.2 ± 1.7†,‡ | 20.9 ± 0.7†,‡ | ||
| PBPC | + | + | 15.9 ± 4.0 | 14.8 ± 2.7 | ND | |
| Transduction Method . | Cell . | Stroma . | Cytokine* . | Transduction Period . | ||
|---|---|---|---|---|---|---|
| 48 h . | 96 h . | 144 h . | ||||
| Supernatant | BMPC | − | + | ND | 1.7 ± 0.6 | ND |
| + | + | ND | 5.6 ± 1.2 | ND | ||
| Cocultivation | BMPC | − | − | 1.0 ± 0.6 | 3.2 ± 1.0 | 5.9 ± 0.9 |
| − | + | 1.6 ± 0.2 | 5.5 ± 0.5 | 6.3 ± 1.2 | ||
| + | − | 11.6 ± 1.5† | 15.3 ± 1.9† | 16.5 ± 1.2† | ||
| + | + | 15.2 ± 1.5† | 23.2 ± 1.7†,‡ | 20.9 ± 0.7†,‡ | ||
| PBPC | + | + | 15.9 ± 4.0 | 14.8 ± 2.7 | ND | |
Transduction efficiency is represented as the percentage of VCR resistant colonies. Repetitive transduction in a coculture system of viral producer and marmoset bone marrow stromal cells facilitated efficient transduction efficiency. Data are expressed as the mean ± SEM from two separate experiments with the supernatant method and from three separate experiments with the cocultivation method.
Abbreviation: ND, not done.
Human SCF (100 ng/mL), IL-3 (20 ng/mL), IL-6 (80 ng/mL).
P < .01 when compared with the group without stromal cells, but with the same cytokine combination during the same transduction period.
P < .05 when compared with the group without cytokines, but with the same stromal cells condition during the same transduction period.