Table 1. Oligonucleotide Primers Used in... | American Society of Hematology

Table 1.

Oligonucleotide Primers Used in RT-PCR

Transcript/ Primer	Genbank Locus (product size)	Primer Sequence	Positions
CD34	HUMCD34HS (229 bp)	5′-ATGGCTTCCTCCTCCCTCCT-3′	2269-2288
		5′-ATCCCTGCTCAACCCCTCTG-3′	2497-2478
DelCD34^*	HUMCD34HS	5′-TTCCTCCTCCCTCCTGCCCCTTCCTTGCTC-3′	2274-2288
			2362-2379
T7DelCD34^*,^†	HUMCD34HS	5′ GGATCCAATACGACTCACTATAGGGACATGGCTTCCTCCTCCCTCCT-3′ T7	2269-2288
pTCD34^*,^‡	HUMCD34HS	5′-dT₂₄-ATCCCTGCTCAACCCCTCTG-3′	2497-2478

Transcript/ Primer	Genbank Locus (product size)	Primer Sequence	Positions
CD34	HUMCD34HS (229 bp)	5′-ATGGCTTCCTCCTCCCTCCT-3′	2269-2288
		5′-ATCCCTGCTCAACCCCTCTG-3′	2497-2478
DelCD34^*	HUMCD34HS	5′-TTCCTCCTCCCTCCTGCCCCTTCCTTGCTC-3′	2274-2288
			2362-2379
T7DelCD34^*,^†	HUMCD34HS	5′ GGATCCAATACGACTCACTATAGGGACATGGCTTCCTCCTCCCTCCT-3′ T7	2269-2288
pTCD34^*,^‡	HUMCD34HS	5′-dT₂₄-ATCCCTGCTCAACCCCTCTG-3′	2497-2478

Primers used for construction of CD34 deletion constructs.

Primer contains the T7 RNA polymerase promoter sequence (underlined).

Primer contains a poly (dT) tail.