Cellular Heterogeneity in Non-cdc Cultures
| Transcripts . | 4 Cells/ Well . | 8-12 Cells/ Well . | 16-24 Cells/ Well . |
|---|---|---|---|
| CD14 | 0/8 | 0/8 | 0/8 |
| CD34 | 8/8 | 8/8 | 8/8 |
| CD36 | 0/10 | 7/12 | 9/10 |
| GPA | 0/8 | 0/8 | 4/11 |
| EpoR | 0/12 | 3/11 | 9/12 |
| β-Globin | 0/8 | 1/10 | 10/12 |
| Transcripts . | 4 Cells/ Well . | 8-12 Cells/ Well . | 16-24 Cells/ Well . |
|---|---|---|---|
| CD14 | 0/8 | 0/8 | 0/8 |
| CD34 | 8/8 | 8/8 | 8/8 |
| CD36 | 0/10 | 7/12 | 9/10 |
| GPA | 0/8 | 0/8 | 4/11 |
| EpoR | 0/12 | 3/11 | 9/12 |
| β-Globin | 0/8 | 1/10 | 10/12 |
Individual CD34+LIN− cells were grown in unilineage erythroid cultures. When wells contained 4, 8 to 12, or 16 to 24 cells/well, individual cells were processed for SIP-RT-PCR as described in Materials and Methods. Data are from one representative experiment. Note the heterogeneity of gene expression among cells isolated from wells containing 8 to 12 and 16 to 24 cells/well.