Sequences and Coordinates of Primers
| Primers . | Sequences . | Coordinates . | PCR Products (bp) . |
|---|---|---|---|
| EBER1 | |||
| E1 | 5′-AGGACCTACGCTGCCCTAGAG-3′ | 6,649 | |
| E1 inner | 5′-AGAGGTTTTGCTAGGGAGG-3′ | 6,664 | |
| E2 | 5′-AAAACATGCGGACCACCAGC-3′ | 6,776 | |
| E2 nested | 5′-GACCACCAGCTGGTACTTG-3′ | 6,767 | 140 |
| Probe | 5′-AGACGTGTGTGGCTGTAG-3′ | 6,683 | |
| RPMS1 | |||
| P1 | 5′-CACGATGTCCTGGTCAGAGTG-3′ | 10,248 | |
| P1 nested | 5′-GGCTTGAGGAATACCTCGTTG-3′ | 10,282 | |
| P2 | 5′-CCTTCGATATGCAGTGTCTG-3′ | 155,847 | |
| P2 nested | 5′-ACCAACGAGGCTGACCTGATC-3′ | 155,801 | 296 |
| Probe | 5′-GCAGATATCCTGCGTCCTC-3′ | 155,781 | |
| BARF0 | |||
| P3 | 5′-GTGAGGGAAATAACCAGGATC-3′ | 157,086 | |
| P3 nested | 5′-CAGGACCAGAATGAGCATGC-3′ | 157,110 | |
| P4 | 5′-GCTTTCCTTTCCGAGTCTGC-3′ | 159,171 | |
| P4 nested | 5′-CTTCTCCTCGGACATCCAGTG-3′ | 159,121 | 237 |
| Probe | 5′-GGAGATGAAACCAGAGAC-3′ | 157,161 | |
| CD23 | |||
| C1 | 5′-GTTGTCAGGGAGTGAGTGC-3′ | ||
| C2 | 5′-GCTCGAAGTTCCTCCAGTTC-3′ | 271 | |
| Probe | 5′-CATCGGGAGAATCCAAGCAG-3′ | ||
| EBNA1 (U-K exon) | |||
| U1 | 5′-GAAGCGTTTCTTGAGCTTCC-3′ | 67,506 | |
| U1 nested | 5′-GTTTGGGAGAGCTGATTCTG-3′ | 67,537 | |
| K1 | 5′-CTCTTCTTTGAGGTCCACTG-3′ | 108,032 | |
| K1 nested | 5′-CTTCTGGTCCAGATGTGTCTC-3′ | 108,002 | 205 |
| Probe | 5′-ATGCCCTGAGACTACTCTCT-3′ | 67,548 |
| Primers . | Sequences . | Coordinates . | PCR Products (bp) . |
|---|---|---|---|
| EBER1 | |||
| E1 | 5′-AGGACCTACGCTGCCCTAGAG-3′ | 6,649 | |
| E1 inner | 5′-AGAGGTTTTGCTAGGGAGG-3′ | 6,664 | |
| E2 | 5′-AAAACATGCGGACCACCAGC-3′ | 6,776 | |
| E2 nested | 5′-GACCACCAGCTGGTACTTG-3′ | 6,767 | 140 |
| Probe | 5′-AGACGTGTGTGGCTGTAG-3′ | 6,683 | |
| RPMS1 | |||
| P1 | 5′-CACGATGTCCTGGTCAGAGTG-3′ | 10,248 | |
| P1 nested | 5′-GGCTTGAGGAATACCTCGTTG-3′ | 10,282 | |
| P2 | 5′-CCTTCGATATGCAGTGTCTG-3′ | 155,847 | |
| P2 nested | 5′-ACCAACGAGGCTGACCTGATC-3′ | 155,801 | 296 |
| Probe | 5′-GCAGATATCCTGCGTCCTC-3′ | 155,781 | |
| BARF0 | |||
| P3 | 5′-GTGAGGGAAATAACCAGGATC-3′ | 157,086 | |
| P3 nested | 5′-CAGGACCAGAATGAGCATGC-3′ | 157,110 | |
| P4 | 5′-GCTTTCCTTTCCGAGTCTGC-3′ | 159,171 | |
| P4 nested | 5′-CTTCTCCTCGGACATCCAGTG-3′ | 159,121 | 237 |
| Probe | 5′-GGAGATGAAACCAGAGAC-3′ | 157,161 | |
| CD23 | |||
| C1 | 5′-GTTGTCAGGGAGTGAGTGC-3′ | ||
| C2 | 5′-GCTCGAAGTTCCTCCAGTTC-3′ | 271 | |
| Probe | 5′-CATCGGGAGAATCCAAGCAG-3′ | ||
| EBNA1 (U-K exon) | |||
| U1 | 5′-GAAGCGTTTCTTGAGCTTCC-3′ | 67,506 | |
| U1 nested | 5′-GTTTGGGAGAGCTGATTCTG-3′ | 67,537 | |
| K1 | 5′-CTCTTCTTTGAGGTCCACTG-3′ | 108,032 | |
| K1 nested | 5′-CTTCTGGTCCAGATGTGTCTC-3′ | 108,002 | 205 |
| Probe | 5′-ATGCCCTGAGACTACTCTCT-3′ | 67,548 |
The coordinates refer to the sequences of Raji (P1 and P1 nested)45 and B95-8,44 respectively. CD23 primers and probe were based on the cDNA sequence previously described.46