Antigenic Composition of CD34+ CB Cells at Initiation of Liquid Cultures and at Various Time Intervals During Ex Vivo Expansion
| Culture Conditions . | CD34 . | CD38 . | CD34+38− . | CD33 . | CD13 . | CD33+13+ . | CD14 . | CD2 . | CD19 . | CD41 . | αGpA . | CD71 . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Start | 91 ± 8.5 | 94 ± 3.1 | 0.95 ± 0.05 | 49 ± 6.4 | 0 | 0 | 0.3 ± 0.03 | 2.0 ± 1.6 | 1.8 ± 1.1 | 0.4 ± 0.2 | 4.1 ± 1.6 | 0 |
| wk 4 | 3.9 ± 0.4 | 76 ± 4.8 | 0.18 ± 0.04 | 96 ± 13 | 94.7 ± 7.8 | 91 ± 11.6 | 59 ± 7.1 | 0.25 ± 0.04 | 0.9 ± 0.2 | 3 ± 1.4 | 5 ± 2 | 2 ± 2 |
| wk 8 | 2.1 ± 0.17 | 27 ± 7 | 0.6 ± 0.15 | 32 ± 9.6 | 1.72 ± 16.4 | 1.7 ± 0.3 | 25 ± 1.4 | 0.12 ± 0.01 | 0.07 ± 0.01 | 0.5 ± 0.01 | 0.3 ± 0.01 | 2.63 ± 1.6 |
| wk 12 | 2.3 ± 1 | 34 ± 11 | 0.46 ± 0.9 | 85.4 ± 2.4 | 7.2 ± 4.3 | 7.1 ± 2.7 | 36.3 ± 2.1 | 0.1 ± 0 | 0.19 ± 0.03 | 0.18 ± 0.6 | 0.56 ± 0.04 | 13.1 ± 2.9 |
| Culture Conditions . | CD34 . | CD38 . | CD34+38− . | CD33 . | CD13 . | CD33+13+ . | CD14 . | CD2 . | CD19 . | CD41 . | αGpA . | CD71 . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Start | 91 ± 8.5 | 94 ± 3.1 | 0.95 ± 0.05 | 49 ± 6.4 | 0 | 0 | 0.3 ± 0.03 | 2.0 ± 1.6 | 1.8 ± 1.1 | 0.4 ± 0.2 | 4.1 ± 1.6 | 0 |
| wk 4 | 3.9 ± 0.4 | 76 ± 4.8 | 0.18 ± 0.04 | 96 ± 13 | 94.7 ± 7.8 | 91 ± 11.6 | 59 ± 7.1 | 0.25 ± 0.04 | 0.9 ± 0.2 | 3 ± 1.4 | 5 ± 2 | 2 ± 2 |
| wk 8 | 2.1 ± 0.17 | 27 ± 7 | 0.6 ± 0.15 | 32 ± 9.6 | 1.72 ± 16.4 | 1.7 ± 0.3 | 25 ± 1.4 | 0.12 ± 0.01 | 0.07 ± 0.01 | 0.5 ± 0.01 | 0.3 ± 0.01 | 2.63 ± 1.6 |
| wk 12 | 2.3 ± 1 | 34 ± 11 | 0.46 ± 0.9 | 85.4 ± 2.4 | 7.2 ± 4.3 | 7.1 ± 2.7 | 36.3 ± 2.1 | 0.1 ± 0 | 0.19 ± 0.03 | 0.18 ± 0.6 | 0.56 ± 0.04 | 13.1 ± 2.9 |
Positive cells (%) of each subpopulation. Mean ± standard deviation (SD) from four to seven separate experiments performed in replicate wells for each growth factor combination at different time points.