Table 5.

Determination of the Cell Number, CFC, LTC-IC, and the Ability to Engraft the BM of NOD/SCID Recipients by Human CB Cells During Stroma-Free Cultures

In CultureInjected/MouseEngraftment
Source of Cells Growth Factors Added Cells (fold increase) CFC (fold increase) LTC-IC (fold increase) Cells CFC LTC-IC% of Human Cells5-150Human CFC/ mouse ×103 5-151
Start   10,000       866.4    1,332   10,000  866.4 1,332  0, 0  0, 0  
      5,000  433 666  0, 0  0, 0  
      2,500  216 333  0, 0  0, 0  
      1,250  108 166  0, 0  0, 0  
4 weeks +IL-6 + SCF +
 FL + MGDF 		3.16 × 106    (316)    37,184      (43)    11,248   (8.4)  3.160 × 106 0.395 × 106 37,184 4,648  11,248 1,456  8.5, 9.9 2.5, 3    15.64, 18.4 7.2, 9.6  
8 weeks  +IL-6 + SCF +
 FL + MGDF 		28.99 × 106  (2,899)   129,464     (149)    56,304 (42)   28.99 × 106 3.60 × 106 129,464 16,183  56,304 7,038  7.3, 18 2.8, 3.6  18.22, 36.72    ND, 34.32  
     1.80 × 106 8,091  3,519  0.7, 1.9  1.34, 3.87  
12 weeks  +IL-6 + SCF +
 FL + MGDF 		109 × 106 (10,900) 2,909,061   (3,358)   382,284 (287)    109 × 106 13.6 × 106 — 363,632  — 47,785  — — 11, 16   34.98, 50.76  
     6.8 × 106 181,816  23,892   8, 6.8  23.44, 44.66 
     3.4 × 106 90,908  11,946 1.4, 0.9  12.6, 19.3  
Start   20,000     2,248      3,632   20,000  3,248  3,632  0, 0  2.08, 0    
      5,000  812  908  0, 0 0, 0  
      2,500  406  454 0, 0  0, 0  
      1,250  203 227  0, 0  0, 0  
7 weeks  FL + MGDF 22 × 106  (1,100)   372,736     (166)    90,112 (25)   22 × 106 2.75 × 106 124,930 15,616  90,112 11,264  6.8, 15   4, 11   29.9, 63.84  8.3, 75.9  
     1.37 × 106 7,808 5,632    0, 2.6     0, 13.4 
 SCF + FL +
 MGDF 		36.4 × 106  (1,820) 381,728   (170)    126,976 (35)  36.40 × 106 4.55 × 106 381,728 47,716  126,976 15,872  7.8, 14.2 5.6, 9    41.87, 46.86 2.08, ND   
     2.27 × 106 23,858 7,936  0.9, 3    2.54, 10.1 
 IL-6 + SCF +
 FL + MGDF 		17.8 × 106    (890)   361,081     (160)   102,400 (28)   17.80 × 106 2.22 × 106 361,081 45,135  102,400 12,800  18, 10.4 4, 6  108.9, 54.16   ND, 23.9  
     1.11 × 106 22,567  6,400    1, 2.1  6.32, 15.6  
12 weeks IL-6 + SCF +
 FL + MGDF 		710 × 106 (35,500) 2,928,640   (1,303)   980,460 (270)    — 88.75 × 106 — 366,080  — 122,580  — — ND, ND  — — ND, ND  
     22.20 × 106 91,520 30,641   11, 6.5  50.16, 30.07 
     11.10 × 106 45,760 15,322  0.6, 3.9  1.87, 6    
     5.50 × 106 22,880  7,661 0.9, 0.5  5.56, 2.3  
In CultureInjected/MouseEngraftment
Source of Cells Growth Factors Added Cells (fold increase) CFC (fold increase) LTC-IC (fold increase) Cells CFC LTC-IC% of Human Cells5-150Human CFC/ mouse ×103 5-151
Start   10,000       866.4    1,332   10,000  866.4 1,332  0, 0  0, 0  
      5,000  433 666  0, 0  0, 0  
      2,500  216 333  0, 0  0, 0  
      1,250  108 166  0, 0  0, 0  
4 weeks +IL-6 + SCF +
 FL + MGDF 		3.16 × 106    (316)    37,184      (43)    11,248   (8.4)  3.160 × 106 0.395 × 106 37,184 4,648  11,248 1,456  8.5, 9.9 2.5, 3    15.64, 18.4 7.2, 9.6  
8 weeks  +IL-6 + SCF +
 FL + MGDF 		28.99 × 106  (2,899)   129,464     (149)    56,304 (42)   28.99 × 106 3.60 × 106 129,464 16,183  56,304 7,038  7.3, 18 2.8, 3.6  18.22, 36.72    ND, 34.32  
     1.80 × 106 8,091  3,519  0.7, 1.9  1.34, 3.87  
12 weeks  +IL-6 + SCF +
 FL + MGDF 		109 × 106 (10,900) 2,909,061   (3,358)   382,284 (287)    109 × 106 13.6 × 106 — 363,632  — 47,785  — — 11, 16   34.98, 50.76  
     6.8 × 106 181,816  23,892   8, 6.8  23.44, 44.66 
     3.4 × 106 90,908  11,946 1.4, 0.9  12.6, 19.3  
Start   20,000     2,248      3,632   20,000  3,248  3,632  0, 0  2.08, 0    
      5,000  812  908  0, 0 0, 0  
      2,500  406  454 0, 0  0, 0  
      1,250  203 227  0, 0  0, 0  
7 weeks  FL + MGDF 22 × 106  (1,100)   372,736     (166)    90,112 (25)   22 × 106 2.75 × 106 124,930 15,616  90,112 11,264  6.8, 15   4, 11   29.9, 63.84  8.3, 75.9  
     1.37 × 106 7,808 5,632    0, 2.6     0, 13.4 
 SCF + FL +
 MGDF 		36.4 × 106  (1,820) 381,728   (170)    126,976 (35)  36.40 × 106 4.55 × 106 381,728 47,716  126,976 15,872  7.8, 14.2 5.6, 9    41.87, 46.86 2.08, ND   
     2.27 × 106 23,858 7,936  0.9, 3    2.54, 10.1 
 IL-6 + SCF +
 FL + MGDF 		17.8 × 106    (890)   361,081     (160)   102,400 (28)   17.80 × 106 2.22 × 106 361,081 45,135  102,400 12,800  18, 10.4 4, 6  108.9, 54.16   ND, 23.9  
     1.11 × 106 22,567  6,400    1, 2.1  6.32, 15.6  
12 weeks IL-6 + SCF +
 FL + MGDF 		710 × 106 (35,500) 2,928,640   (1,303)   980,460 (270)    — 88.75 × 106 — 366,080  — 122,580  — — ND, ND  — — ND, ND  
     22.20 × 106 91,520 30,641   11, 6.5  50.16, 30.07 
     11.10 × 106 45,760 15,322  0.6, 3.9  1.87, 6    
     5.50 × 106 22,880  7,661 0.9, 0.5  5.56, 2.3  

The value in parentheses represents the fold increase compared with input values.

F5-150

Each value represents the percent of human CD45+, CD71+, and α-GPA+ cells in the BM of each individual mouse.

F5-151

As explained in Table 4. Expansion studies were performed in quadruplicate. Two mice were injected with each cell dose as indicated.

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