ASOs and Double-Stranded EMSA Oligonucleotides for Each Polymorphic Site
| SNP . | ASO and ds EMSA oligo* . | ASO Tm (°C) . |
|---|---|---|
| 5′-ACGCCATT(C/T)TCCTGCCT-3′ | ||
| −1234C/T | 5′-GGGTTCACGCCATT(C/T)TCCTGCCT-3′ | 54/52 |
| 3′-GTGCGGTAA(G/A)AGGACGGAGTCGG-5′ | ||
| 5′-CCGCCACC(A/G)CGCCTGGC-3′ | ||
| −1185A/G | 5′-TACAGGCGCCCGCCACC(A/G)CGCCTG-3′ | 62/64 |
| 3′-CGCGGGCGGTGG(T/C)GCGGACCGATT-5′ | ||
| 5′-GAACCACC(G/A)TGCCCGGC-3′ | ||
| −1051G/A | 5′-GCGTGAACCACC(G/A)TGCCCGGCCC-3′ | 60/58 |
| 3′-TTGGTGG(C/T)ACGGGCCGGGGGGTT-5′ |
| SNP . | ASO and ds EMSA oligo* . | ASO Tm (°C) . |
|---|---|---|
| 5′-ACGCCATT(C/T)TCCTGCCT-3′ | ||
| −1234C/T | 5′-GGGTTCACGCCATT(C/T)TCCTGCCT-3′ | 54/52 |
| 3′-GTGCGGTAA(G/A)AGGACGGAGTCGG-5′ | ||
| 5′-CCGCCACC(A/G)CGCCTGGC-3′ | ||
| −1185A/G | 5′-TACAGGCGCCCGCCACC(A/G)CGCCTG-3′ | 62/64 |
| 3′-CGCGGGCGGTGG(T/C)GCGGACCGATT-5′ | ||
| 5′-GAACCACC(G/A)TGCCCGGC-3′ | ||
| −1051G/A | 5′-GCGTGAACCACC(G/A)TGCCCGGCCC-3′ | 60/58 |
| 3′-TTGGTGG(C/T)ACGGGCCGGGGGGTT-5′ |
Synthesized by Cortec DNA Service Laboratories.