Table 1. Production of Mast Cells by... | American Society of Hematology

Table 1.

Production of Mast Cells by CD34⁺CD38⁺c-kit⁺ Cells and CD34⁺CD38⁻c-kit⁺ Cells Under Stimulation With SCF Plus TPO

CD38/c-kit	Stimuli	Colony Type						Total
CD38/c-kit	Stimuli	n	nm	mast	nmast	MK	U	Total
+/+	SCF	0	0	0	1	2	1	4
	TPO	0	0	0	0	3	0	3
	FL	0	0	0	0	0	1	1
	SCF + TPO	0	0	0	8	2	7	17
	SCF + FL	1	1	0	1	2	6	11
	TPO + FL	0	1	0	0	2	0	3
	SCF + TPO + FL	4	2	0	8	1	8	23
−/+	SCF	0	0	0	0	0	1	1
	TPO	0	0	0	0	1	0	1
	FL	0	0	0	0	0	0	0
	SCF + TPO	0	0	0	4	2	4	10
	SCF + FL	5	1	0	1	3	6	16
	TPO + FL	0	0	0	0	2	12	14
	SCF + TPO + FL	10	1	0	4	0	6	21
+/−	SCF	0	0	0	0	0	1	1
	TPO	0	0	0	0	1	0	1
	FL	0	0	0	0	0	0	0
	SCF + TPO	0	0	0	0	2	0	2
	SCF + FL	0	0	0	0	0	0	0
	TPO + FL	0	0	0	0	2	0	2
	SCF + TPO + FL	0	0	0	0	0	0	0
−/−	SCF	0	0	0	0	0	0	0
	TPO	0	0	0	0	0	0	0
	FL	0	0	0	0	0	0	0
	SCF + TPO	0	0	0	0	0	0	0
	SCF + FL	0	0	0	0	0	0	0
	TPO + FL	0	0	0	0	0	0	0
	SCF + TPO + FL	0	1	0	1	0	0	2

CD38/c-kit	Stimuli	Colony Type						Total
CD38/c-kit	Stimuli	n	nm	mast	nmast	MK	U	Total
+/+	SCF	0	0	0	1	2	1	4
	TPO	0	0	0	0	3	0	3
	FL	0	0	0	0	0	1	1
	SCF + TPO	0	0	0	8	2	7	17
	SCF + FL	1	1	0	1	2	6	11
	TPO + FL	0	1	0	0	2	0	3
	SCF + TPO + FL	4	2	0	8	1	8	23
−/+	SCF	0	0	0	0	0	1	1
	TPO	0	0	0	0	1	0	1
	FL	0	0	0	0	0	0	0
	SCF + TPO	0	0	0	4	2	4	10
	SCF + FL	5	1	0	1	3	6	16
	TPO + FL	0	0	0	0	2	12	14
	SCF + TPO + FL	10	1	0	4	0	6	21
+/−	SCF	0	0	0	0	0	1	1
	TPO	0	0	0	0	1	0	1
	FL	0	0	0	0	0	0	0
	SCF + TPO	0	0	0	0	2	0	2
	SCF + FL	0	0	0	0	0	0	0
	TPO + FL	0	0	0	0	2	0	2
	SCF + TPO + FL	0	0	0	0	0	0	0
−/−	SCF	0	0	0	0	0	0	0
	TPO	0	0	0	0	0	0	0
	FL	0	0	0	0	0	0	0
	SCF + TPO	0	0	0	0	0	0	0
	SCF + FL	0	0	0	0	0	0	0
	TPO + FL	0	0	0	0	0	0	0
	SCF + TPO + FL	0	1	0	1	0	0	2

To determine which subpopulations of CD34⁺ BM cells were responsible for the SCF + TPO–dependent mast cell production, we divided CD34⁺ cells into 4 subsets, as presented in Fig 6. Single-cell sorting was performed in all of the subsets by a FACStar^plus flow cytometer, and the sorted individual cells were incubated with SCF, TPO, or FL, alone or in combination, for 3 weeks.

Abbreviations: n, neutrophil; m, macrophage; mast, mast cell; MK, megakaryocyte; U, undifferentiated cell.

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