Phycoerythrin-Conjugated rhG-CSF (PE-G-CSF) Binding to WEHI-3B D+ Cells Transfected With Full-Length or Mutant G-CSFR Expression Plasmids
| Cell Line* . | Relative Fluorescence Intensity† . | ||
|---|---|---|---|
| Control . | PE-G-CSF . | PE-G-CSF + rhG-CSF . | |
| D+V | 1.00 | 1.38 | 1.05 |
| D+GR95 | 1.00 | 2.99 | 0.96 |
| D+GR-A | 1.00 | 3.86 | 1.00 |
| D+GR-B | 1.00 | 4.92 | 1.08 |
| D+GR-C | 1.00 | 4.24 | 1.00 |
| D+GR-D | 1.00 | 3.93 | 1.06 |
| D+GR-E | 1.00 | 5.74 | 0.96 |
| D+GR-F | 1.00 | 17.89 | 1.02 |
| Cell Line* . | Relative Fluorescence Intensity† . | ||
|---|---|---|---|
| Control . | PE-G-CSF . | PE-G-CSF + rhG-CSF . | |
| D+V | 1.00 | 1.38 | 1.05 |
| D+GR95 | 1.00 | 2.99 | 0.96 |
| D+GR-A | 1.00 | 3.86 | 1.00 |
| D+GR-B | 1.00 | 4.92 | 1.08 |
| D+GR-C | 1.00 | 4.24 | 1.00 |
| D+GR-D | 1.00 | 3.93 | 1.06 |
| D+GR-E | 1.00 | 5.74 | 0.96 |
| D+GR-F | 1.00 | 17.89 | 1.02 |
WEHI-3B D+ cells were transfected with vector alone, the full-length G-CSFR expression plasmid, or mutant G-CSFR expression plasmids to generate D+V, D+GR95, D+GR-A, D+GR-B, D+GR-C, D+GR-D, D+GR-E, and D+GR-F cells, respectively.
Transfected cells were stained with PE-G-CSF with or without preincubation in a 10-fold molar excess of unconjugated cytokine. Controls are transfected cells stained with phycoerythrin-conjugated streptavidin. The fluorescence intensity of controls was defined as 1.00, and the numbers indicate the representative fold change in fluorescence intensity compared with controls.