Differentiation of WEHI-3B D+ Cells Transfected With Full-Length or Mutant G-CSFR Expression Plasmids as Determined by NBT Positivity After G-CSF Treatment
| Cell Line . | NBT Positivity (%)3-150 . | |
|---|---|---|
| Untreated . | G-CSF–Treated . | |
| D+V | 2.3 ± 1.3 | 11.3 ± 3.6 |
| D+GR95 | 9.3 ± 1.9 | 88.3 ± 5.0 |
| D+GR-A | 5.3 ± 2.8 | 69.0 ± 4.7 |
| D+GR-B | 1.5 ± 0.9 | 41.0 ± 9.9 |
| D+GR-C | 6.5 ± 4.6 | 70.3 ± 4.7 |
| D+GR-D | 5.5 ± 3.4 | 58.0 ± 7.4 |
| D+GR-E | 1.5 ± 0.9 | 2.5 ± 1.1 |
| D+GR-F | 1.8 ± 0.8 | 1.8 ± 0.4 |
| Cell Line . | NBT Positivity (%)3-150 . | |
|---|---|---|
| Untreated . | G-CSF–Treated . | |
| D+V | 2.3 ± 1.3 | 11.3 ± 3.6 |
| D+GR95 | 9.3 ± 1.9 | 88.3 ± 5.0 |
| D+GR-A | 5.3 ± 2.8 | 69.0 ± 4.7 |
| D+GR-B | 1.5 ± 0.9 | 41.0 ± 9.9 |
| D+GR-C | 6.5 ± 4.6 | 70.3 ± 4.7 |
| D+GR-D | 5.5 ± 3.4 | 58.0 ± 7.4 |
| D+GR-E | 1.5 ± 0.9 | 2.5 ± 1.1 |
| D+GR-F | 1.8 ± 0.8 | 1.8 ± 0.4 |
Transfected cells were treated for 3 days with 10 ng/mL of rhG-CSF.
Each value is the average of 3 independent experiments ± SD.